DNA FINGERPRINTING OF SWEET CHERRY CULTIVARS USING RAPD MARKERS

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MOSHIRI F.; SHOKOUHIFAR F.; BAGHERI A.A.R.

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Paper Information

Journal: MODERN GENETICS JOURNAL (MGJ) Year:2008 | Volume:2 | Issue:4 Page(s): 39-48

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Title

DNA FINGERPRINTING OF SWEET CHERRY CULTIVARS USING RAPD MARKERS

Author(s)

MOSHIRI F. | SHOKOUHIFAR F. | BAGHERI A.A.R. | Issue Writer Certificate

Keywords

DNA FINGERPRINTINGQ2

RAPDQ3

SWEET CHERRYQ3

UPGMAQ2

Abstract

Fine identification of cherry genotypes is essential and useful for breeding programs and germplasm preservation especially in fruit trees. RAPD is one of the extremely used techniques for germplasm DNA FINGERPRINTING. In this study, genomic DNA of ten Iranian and two foreign cherry cultivars were analyzed using twenty-five RAPD primers.Only 14 of primers revealed polymorphic patterns between the cultivars and about 50 polymorphic bands were amplified. The primer UBC138 revealed highest polymorphic loci, but genetic index ofUBC7 (0.82) was more than the others. The genetic distance among cultivars was estimated based on Nei distance index and cluster analysis using UPGMA method.The genetic distance values ranged from 0.32 to 0.88. The lowest distance obtained was found between the cultivars 4 and 10 and the highest distance obtained between the cultivars 1 and 6. The cultivars are discriminated using 12 RAPDs. The cultivars 1,2,3,5 and 6 could be identified using five unique markers and the rest of cultivars should be discriminated with combination of two markers.

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ارزیابی صفات کمی و کیفی میوه و ویژگیهای رویشی ارقام گیلاس و تعیین رابطه همبستگی بین برخی از این صفات

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APA: Copy

MOSHIRI, F., SHOKOUHIFAR, F., & BAGHERI, A.A.R.. (2008). DNA FINGERPRINTING OF SWEET CHERRY CULTIVARS USING RAPD MARKERS. MODERN GENETICS JOURNAL (MGJ), 2(4), 39-48. SID. https://sid.ir/paper/116530/en

Vancouver: Copy

MOSHIRI F., SHOKOUHIFAR F., BAGHERI A.A.R.. DNA FINGERPRINTING OF SWEET CHERRY CULTIVARS USING RAPD MARKERS. MODERN GENETICS JOURNAL (MGJ)[Internet]. 2008;2(4):39-48. Available from: https://sid.ir/paper/116530/en

IEEE: Copy

F. MOSHIRI, F. SHOKOUHIFAR, and A.A.R. BAGHERI, “DNA FINGERPRINTING OF SWEET CHERRY CULTIVARS USING RAPD MARKERS,” MODERN GENETICS JOURNAL (MGJ), vol. 2, no. 4, pp. 39–48, 2008, [Online]. Available: https://sid.ir/paper/116530/en

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