STUDY OF RESISTANCE RELATED GENE EXPRESSION PATTERN TO SEPTORIA TRITICI BLOTCH (STB) IN WHEAT (TRITICUM AESTIVUM)

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HABIBI M.; MIRAKHORLI N.; SHIRAN B.; MARDI M.

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Journal: MODERN GENETICS JOURNAL (MGJ) Year:2013 | Volume:8 | Issue:2 (33) Page(s): 149-158

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Title

STUDY OF RESISTANCE RELATED GENE EXPRESSION PATTERN TO SEPTORIA TRITICI BLOTCH (STB) IN WHEAT (TRITICUM AESTIVUM)

Author(s)

HABIBI M. | MIRAKHORLI N. | SHIRAN B. | MARDI M. | Issue Writer Certificate

Keywords

GENE EXPRESSIONQ2

MYCOSPHAERELLA GRAMINICOLAQ2

PR GENESQ2

RT-PCRQ2

TRITICUM AESTIVUMQ2

Abstract

Septoria tritici bloth (STB), caused by MYCOSPHAERELLA GRAMINICOLA (anamorph: septoria tritici) is one of the most devastating diseases of wheat (Triticum aestivum) and has a major impact on yields Worldwide. During the previous expression-profiling experiment, in addition to major resistant genes to STB (stb1-15), some defense-related genes have bean identified. To study expression pattern of the five defense-related genes including PR-1, Chitinase, Peroxidase, PR-5 and Protease inhibitor, over time, their level of expression were measured at 8 time points, from 0h to 6 days after inoculation in Wangshuibai as a resistant wheat cultivar and Falat as a susceptible one by semi quantitative (RT-PCR). The result showed that, infection of wheat by M. graminicola induced changes in expression of all five genes in both resistant and susceptible cultivar over time. Expression pattern of these genes indicated that all of five genes in resistant cultivar induced earlier than in susceptible cultivare. The RT-PCR analysis confirmed that expression of these genes in control resistance cultivare are much higher than susceptible cultivar in majority of the times during the 6-days time course. Thus, according this results can be concluded that these genes, along the main genes, increase and maintain resistance to Septoria tritici blotch in wheat.

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APA: Copy

HABIBI, M., MIRAKHORLI, N., SHIRAN, B., & MARDI, M.. (2013). STUDY OF RESISTANCE RELATED GENE EXPRESSION PATTERN TO SEPTORIA TRITICI BLOTCH (STB) IN WHEAT (TRITICUM AESTIVUM). MODERN GENETICS JOURNAL (MGJ), 8(2 (33)), 149-158. SID. https://sid.ir/paper/116601/en

Vancouver: Copy

HABIBI M., MIRAKHORLI N., SHIRAN B., MARDI M.. STUDY OF RESISTANCE RELATED GENE EXPRESSION PATTERN TO SEPTORIA TRITICI BLOTCH (STB) IN WHEAT (TRITICUM AESTIVUM). MODERN GENETICS JOURNAL (MGJ)[Internet]. 2013;8(2 (33)):149-158. Available from: https://sid.ir/paper/116601/en

IEEE: Copy

M. HABIBI, N. MIRAKHORLI, B. SHIRAN, and M. MARDI, “STUDY OF RESISTANCE RELATED GENE EXPRESSION PATTERN TO SEPTORIA TRITICI BLOTCH (STB) IN WHEAT (TRITICUM AESTIVUM),” MODERN GENETICS JOURNAL (MGJ), vol. 8, no. 2 (33), pp. 149–158, 2013, [Online]. Available: https://sid.ir/paper/116601/en

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