IDENTIFICATION OF MOLECULAR MARKERS LINKED TO WA-CYTOPLASMIC MALE STERILITY IN RICE AND DEVELOPMENT OF SPECIFIC SCAR MARKERS

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AHMADIKHAH A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: MODERN GENETICS JOURNAL (MGJ) Year:2009 | Volume:3 | Issue:4 Page(s): 65-74

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Title

IDENTIFICATION OF MOLECULAR MARKERS LINKED TO WA-CYTOPLASMIC MALE STERILITY IN RICE AND DEVELOPMENT OF SPECIFIC SCAR MARKERS

Author(s)

AHMADIKHAH A. | Issue Writer Certificate

Keywords

RICEQ2

CMSQ3

MOLECULAR MARKERSQ2

SCARQ3

RAPDQ3

Abstract

In this research four CMS lines and four their maintainer counterparts were used for molecular studies. DNA from iso-cytoplasmic CMS and maintainer lines was amplified using 35 RAPD primers. PCR with these RAPDs showed that 5 primers could produce polymorphic bands between CMS line Neda-A and its maintainer Neda-B. To confirm precise association of the identified markers to male sterile or normal cytoplasm, DNA from further 6 CMS and respective maintainer lines were screened using the 5 polymorphic primers, among which only two primers OPC05 and OPH03 could amplify CMSspecific bands. The primers produced ~500bp and ~1900bp bands, respectively. The ~1900bp band was successfully cloned in pGEM-T vector and then sequenced. Several new primers were designed based on the sequenced fragment, and were used for detecting polymorphism between CMS and maintainer line. PCR with these SCAR primers indicated that a primer combination (SCARmt01-F – SCARmt01-R) could produce polymorphism between CMS and maintainer line. Also the combination of RAPD primers was resulted into the detection of polymorphism between CMS and maintainer line when OPA01 were combined with OPB04. Produced band by this primer combination was cloned, sequenced, and new three pairs of SCAR primers were designed according to the sequenced fragment. PCR with these specific primers was resulted into identification of a new co-dominant, specific SCAR marker with the ability of discriminating between CMS and maintainer line. The use of identified specific markers can accelerate progress of hybrid RICE production program.

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APA: Copy

AHMADIKHAH, A.. (2009). IDENTIFICATION OF MOLECULAR MARKERS LINKED TO WA-CYTOPLASMIC MALE STERILITY IN RICE AND DEVELOPMENT OF SPECIFIC SCAR MARKERS. MODERN GENETICS JOURNAL (MGJ), 3(4), 65-74. SID. https://sid.ir/paper/116637/en

Vancouver: Copy

AHMADIKHAH A.. IDENTIFICATION OF MOLECULAR MARKERS LINKED TO WA-CYTOPLASMIC MALE STERILITY IN RICE AND DEVELOPMENT OF SPECIFIC SCAR MARKERS. MODERN GENETICS JOURNAL (MGJ)[Internet]. 2009;3(4):65-74. Available from: https://sid.ir/paper/116637/en

IEEE: Copy

A. AHMADIKHAH, “IDENTIFICATION OF MOLECULAR MARKERS LINKED TO WA-CYTOPLASMIC MALE STERILITY IN RICE AND DEVELOPMENT OF SPECIFIC SCAR MARKERS,” MODERN GENETICS JOURNAL (MGJ), vol. 3, no. 4, pp. 65–74, 2009, [Online]. Available: https://sid.ir/paper/116637/en

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