EXPRESSION OF OMPF GENE IN E. COLI MUTANTS RESISTANT TO CIPROFLOXACIN AND TETRACYCLINE

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HEIDARI F.; POURAHMAD R.; SHAREGHI B.

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ISSN: 2588-4824

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Journal: MODERN GENETICS JOURNAL (MGJ) Year:2015 | Volume:10 | Issue:1 Page(s): 123-128

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Title

EXPRESSION OF OMPF GENE IN E. COLI MUTANTS RESISTANT TO CIPROFLOXACIN AND TETRACYCLINE

Author(s)

HEIDARI F. | POURAHMAD R. | SHAREGHI B. | Issue Writer Certificate

Keywords

E. COLIQ2

GYRA MARR MUTANTQ2

MULTIPLE DRUG RESISTANCEQ2

OMPFQ2

QPCRQ2

Abstract

OmpF is one of outer membrane proteins of gram negative bacteria that facilitates uptake of fluoroquinolones, including ciprofloxacin inside cell. Thus, regulating of OMPF expression is an effective mechanism of antimicrobial resistance. The aim of this research was assessment of OMPF expression in gyrA and gyrA marR double mutants. Expression of OMPF gene in above mutants with low to intermediate MICs for ciprofloxacin and tetracycline in comparison with wild type, MG1655 was assessed by real time PCR. Statistical analysis of results obtained from real time PCR reaction did not show significant difference between expression of OMPF in mutants and wild type. It was concluded that low and intermediate resistance to ciprofloxacin and tetracycline in investigated mutants did not cause decreased OMPF expression.

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APA: Copy

HEIDARI, F., POURAHMAD, R., & SHAREGHI, B.. (2015). EXPRESSION OF OMPF GENE IN E. COLI MUTANTS RESISTANT TO CIPROFLOXACIN AND TETRACYCLINE. MODERN GENETICS JOURNAL (MGJ), 10(1), 123-128. SID. https://sid.ir/paper/116652/en

Vancouver: Copy

HEIDARI F., POURAHMAD R., SHAREGHI B.. EXPRESSION OF OMPF GENE IN E. COLI MUTANTS RESISTANT TO CIPROFLOXACIN AND TETRACYCLINE. MODERN GENETICS JOURNAL (MGJ)[Internet]. 2015;10(1):123-128. Available from: https://sid.ir/paper/116652/en

IEEE: Copy

F. HEIDARI, R. POURAHMAD, and B. SHAREGHI, “EXPRESSION OF OMPF GENE IN E. COLI MUTANTS RESISTANT TO CIPROFLOXACIN AND TETRACYCLINE,” MODERN GENETICS JOURNAL (MGJ), vol. 10, no. 1, pp. 123–128, 2015, [Online]. Available: https://sid.ir/paper/116652/en

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