INVESTIGATION ON EFFECT OF SODIUM AZIDE IN HAIRY ROOT CULTURE OF ARTEMISIA VULGARIS BY ISSR AND RAPD MARKERS

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YARYZADEH K.; HOSSEINI R.; ALBORZIAN DEHSHEIKH A.

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Journal: MODERN GENETICS JOURNAL (MGJ) Year:2015 | Volume:9 | Issue:4 (39) Page(s): 483-492

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Title

INVESTIGATION ON EFFECT OF SODIUM AZIDE IN HAIRY ROOT CULTURE OF ARTEMISIA VULGARIS BY ISSR AND RAPD MARKERS

Author(s)

YARYZADEH K. | HOSSEINI R. | ALBORZIAN DEHSHEIKH A. | Issue Writer Certificate

Keywords

HAIRY ROOTQ2

ISSRQ2

MUTATIONQ2

RAPDQ2

SODIUM AZIDEQ2

Abstract

Sodium azide (NaN3) is a mutagenic agent that causes point MUTATIONs in the genome. Induced mutagenesis is a useful method for the improvement of a character(s) in the plants. Artemisia vulgaris plants which called Berenjasf in Iran is contained useful secondary metabolites. The production of mutant colonies of HAIRY ROOT cultures of this plant could be a suitable strategy for increasing secondary metabolites. In this study, HAIRY ROOTs were induced in in vitro condition using strain A4 of Agrobacterium rhizogenes. Mutagenesis using NaN3 at 5 concentrations (1-5 mM), was applied. The culture medium without SODIUM AZIDE was used as the control. To monitor mutagenesis, PCR products obtained from ISSR and RAPD markers were analyzed on agarose gel. Results were analyzed by the NTYSIS software. Results showed that four out of five concentrations of SODIUM AZIDE caused mutagenesis. Only 1mM SODIUM AZIDE did not show mutagenesis in the samples. The lowest similarity coefficient compared to the control was observed 21%, by RAPD and 33% by ISSR, both of which were obtained by 2 mM SODIUM AZIDE concentration.

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APA: Copy

YARYZADEH, K., HOSSEINI, R., & ALBORZIAN DEHSHEIKH, A.. (2015). INVESTIGATION ON EFFECT OF SODIUM AZIDE IN HAIRY ROOT CULTURE OF ARTEMISIA VULGARIS BY ISSR AND RAPD MARKERS. MODERN GENETICS JOURNAL (MGJ), 9(4 (39)), 483-492. SID. https://sid.ir/paper/116690/en

Vancouver: Copy

YARYZADEH K., HOSSEINI R., ALBORZIAN DEHSHEIKH A.. INVESTIGATION ON EFFECT OF SODIUM AZIDE IN HAIRY ROOT CULTURE OF ARTEMISIA VULGARIS BY ISSR AND RAPD MARKERS. MODERN GENETICS JOURNAL (MGJ)[Internet]. 2015;9(4 (39)):483-492. Available from: https://sid.ir/paper/116690/en

IEEE: Copy

K. YARYZADEH, R. HOSSEINI, and A. ALBORZIAN DEHSHEIKH, “INVESTIGATION ON EFFECT OF SODIUM AZIDE IN HAIRY ROOT CULTURE OF ARTEMISIA VULGARIS BY ISSR AND RAPD MARKERS,” MODERN GENETICS JOURNAL (MGJ), vol. 9, no. 4 (39), pp. 483–492, 2015, [Online]. Available: https://sid.ir/paper/116690/en

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