DGGE TECHNIQUE APPLICATION TO STUDY GENETIC DIVERSITY OF NA+/K+ ATPASE GENE AMONG ARTEMIA POPULATIONS

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MOOSAVI TOOMATARI G.; MANAFFAR R.; ZARE S.

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Journal: MODERN GENETICS JOURNAL (MGJ) Year:2015 | Volume:9 | Issue:4 (39) Page(s): 411-418

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Title

DGGE TECHNIQUE APPLICATION TO STUDY GENETIC DIVERSITY OF NA+/K+ ATPASE GENE AMONG ARTEMIA POPULATIONS

Author(s)

MOOSAVI TOOMATARI G. | MANAFFAR R. | ZARE S. | Issue Writer Certificate

Keywords

ARTEMIAQ2

DENATURING GELQ2

DGGEQ2

NA+/K+ ATPASEQ2

RFLPQ2

Abstract

Denaturing gradient gel electrophoresis (DGGE) is a molecular fingerprinting method capable of detecting differences among double-stranded DNA fragments according to melting temperature. In present study, genetic diversity of exon-7 of Na+/K+ ATPase among several ARTEMIA populations were studied. The study was conducted with six different ARTEMIA populations as A. urmiana, Parthenogenetic ARTEMIA Maharlu Lake, Parthenogenetic ARTEMIA Urmia Lake, Parthenogenetic ARTEMIA Incheh Lake from Iran, and A. franciscana and A. sinica using RFLP, DGGE and sequencing methods. After DNA extraction, 280 bp fragment of exon-7 of this gene was amplified using genus specific primer. Due to unsuccessful digestion by 8 different restriction digestion enzymes in RFLP analysis, the exon was analyzed by DGGE method. According to recent electrophoresis several new pattern and polymorphisms were detected by DGGE, Which were not identified by RFLP. Authenticity of the found polymorphism was checked by the fragment sequencing. The calculated genetic distance among the ARTEMIA populations, based on the nucleotide diversity, proposed this theory that probably exon-7 is an important part of Na+/K+ ATPase gene in ARTEMIA genus which play an important role in ARTEMIA speciation.

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APA: Copy

MOOSAVI TOOMATARI, G., MANAFFAR, R., & ZARE, S.. (2015). DGGE TECHNIQUE APPLICATION TO STUDY GENETIC DIVERSITY OF NA⁺/K⁺ ATPASE GENE AMONG ARTEMIA POPULATIONS. MODERN GENETICS JOURNAL (MGJ), 9(4 (39)), 411-418. SID. https://sid.ir/paper/116692/en

Vancouver: Copy

MOOSAVI TOOMATARI G., MANAFFAR R., ZARE S.. DGGE TECHNIQUE APPLICATION TO STUDY GENETIC DIVERSITY OF NA⁺/K⁺ ATPASE GENE AMONG ARTEMIA POPULATIONS. MODERN GENETICS JOURNAL (MGJ)[Internet]. 2015;9(4 (39)):411-418. Available from: https://sid.ir/paper/116692/en

IEEE: Copy

G. MOOSAVI TOOMATARI, R. MANAFFAR, and S. ZARE, “DGGE TECHNIQUE APPLICATION TO STUDY GENETIC DIVERSITY OF NA⁺/K⁺ ATPASE GENE AMONG ARTEMIA POPULATIONS,” MODERN GENETICS JOURNAL (MGJ), vol. 9, no. 4 (39), pp. 411–418, 2015, [Online]. Available: https://sid.ir/paper/116692/en

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