EVALUATION OF GENETIC DIVERSITY OF SOME CULTIVATED BARLEY (HORDEUM VULGARE L.) LINES BY AFLP MARKERS

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SHIRI A.; RAMEZANPOUR S.S.; SOLTANLOO H.; KALATEH ARABI M.; KIA SH.

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Journal: MODERN GENETICS JOURNAL (MGJ) Year:2015 | Volume:9 | Issue:4 (39) Page(s): 429-438

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Title

EVALUATION OF GENETIC DIVERSITY OF SOME CULTIVATED BARLEY (HORDEUM VULGARE L.) LINES BY AFLP MARKERS

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Keywords

BARLEYQ1

CLUSTER ANALYSISQ2

PRINCIPLE COORDINATE ANALYSISQ3

MOLECULAR MARKER AFLPQ2

Abstract

BARLEY is one of the important cereals around the world which is used as food and feed. It is belonged to Gramineae and includes cultivated and wild types. Genetic diversity losses resulted to sever vulnerability of cultivated crops against environmental stresses, pests and diseases which cause yield reduction. In order to study genetic diversity, 64 BARLEY lines were evaluated by AFLP molecular markers. The lines were cultivated in Gorgan Agricultural research station under a complete block design arrangement with three replications. The goal of this study was molecular evaluation and determination of genetic diversity between studied BARLEY lines and using the resulted information in parent selection for crosses and production of suitable populations in subsequent generations. Eight AFLP primer combinations produced 749 bands which 93 band were polymorphic. The maximum and minimum polymorphic bands was produced by E-ACA/M-CTA (16 bands) and E-ACA/M-CTC (7 bands) primer combinations, respectively. E-ACA/M-CAA primer combination showed the highest PIC value, thus it can be used for determination between much closed BARLEY genotypes. CLUSTER ANALYSIS of molecular traits based on Jaccard similarity coefficient, divided studied lines into 7 clusters. PRINCIPLE COORDINATE ANALYSIS confirmed the CLUSTER ANALYSIS results. The highest genetic distance or lowest genetic similarity was between line 41 and Sahra (land race). Therefore these two genotypes would be good source in breeding programs to cross and production of population with high genetic diversity.

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APA: Copy

SHIRI, A., RAMEZANPOUR, S.S., SOLTANLOO, H., KALATEH ARABI, M., & KIA, SH.. (2015). EVALUATION OF GENETIC DIVERSITY OF SOME CULTIVATED BARLEY (HORDEUM VULGARE L.) LINES BY AFLP MARKERS. MODERN GENETICS JOURNAL (MGJ), 9(4 (39)), 429-438. SID. https://sid.ir/paper/116693/en

Vancouver: Copy

SHIRI A., RAMEZANPOUR S.S., SOLTANLOO H., KALATEH ARABI M., KIA SH.. EVALUATION OF GENETIC DIVERSITY OF SOME CULTIVATED BARLEY (HORDEUM VULGARE L.) LINES BY AFLP MARKERS. MODERN GENETICS JOURNAL (MGJ)[Internet]. 2015;9(4 (39)):429-438. Available from: https://sid.ir/paper/116693/en

IEEE: Copy

A. SHIRI, S.S. RAMEZANPOUR, H. SOLTANLOO, M. KALATEH ARABI, and SH. KIA, “EVALUATION OF GENETIC DIVERSITY OF SOME CULTIVATED BARLEY (HORDEUM VULGARE L.) LINES BY AFLP MARKERS,” MODERN GENETICS JOURNAL (MGJ), vol. 9, no. 4 (39), pp. 429–438, 2015, [Online]. Available: https://sid.ir/paper/116693/en

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