EVALUATION OF GENETIC VARIATION IN SOME IRANIAN ORIENTAL AND SEMI ORIENTAL TOBACCO GERMPLASMS BY USING SIMPLE SEQUENCE REPEAT MARKERS

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VAGHARI AZAR E.; HATAMI MALEKI H.; BASIRNIA A.; AHMADI D.; DARVISHZADEH R.

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Journal: MODERN GENETICS JOURNAL (MGJ) Year:2015 | Volume:9 | Issue:4 (39) Page(s): 517-523

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Title

EVALUATION OF GENETIC VARIATION IN SOME IRANIAN ORIENTAL AND SEMI ORIENTAL TOBACCO GERMPLASMS BY USING SIMPLE SEQUENCE REPEAT MARKERS

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Keywords

GENETIC DIVERSITYQ2

JACCARD'S SIMILARITYQ2

SSR MARKERQ2

TOBACCOQ2

UPGMA CLUSTERING ALGORITHMQ2

Abstract

Characterization of GENETIC DIVERSITY has long been a major goal in TOBACCO breeding programs. In this research, the GENETIC DIVERSITY of TOBACCO germplasm including 90 oriental and semi oriental TOBACCO genotypes was investigated using simple sequence repeat markers. Herein, 26 microsatellite primer pairs out of 278 microsatellite primer pairs were able to clarify existence polymorphism in individuals DNA. Results of fingerprinting of genotypes using 26 microsatellite primer pairs revealed 67 alleles. The mean number of alleles per locus (na) and the effective allele number (ne) were 2.481 and 2.159 respectively. The expected heterozygosity ranged from 0.326 to 0.662 with an average of 0.512. Based on JACCARD'S SIMILARITY coefficient, similarity values among genotypes were varied among 0.04 to 0.78 which imply the existence of GENETIC DIVERSITY in studied germplasm. Classification of TOBACCO germplasm via UPGMA CLUSTERING ALGORITHM distinguished it into 4 separate groups. Results of cluster analysis manifested that the resultant classification did not accompany with the geographical distribution of genotypes.

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APA: Copy

VAGHARI AZAR, E., HATAMI MALEKI, H., BASIRNIA, A., AHMADI, D., & DARVISHZADEH, R.. (2015). EVALUATION OF GENETIC VARIATION IN SOME IRANIAN ORIENTAL AND SEMI ORIENTAL TOBACCO GERMPLASMS BY USING SIMPLE SEQUENCE REPEAT MARKERS. MODERN GENETICS JOURNAL (MGJ), 9(4 (39)), 517-523. SID. https://sid.ir/paper/116694/en

Vancouver: Copy

VAGHARI AZAR E., HATAMI MALEKI H., BASIRNIA A., AHMADI D., DARVISHZADEH R.. EVALUATION OF GENETIC VARIATION IN SOME IRANIAN ORIENTAL AND SEMI ORIENTAL TOBACCO GERMPLASMS BY USING SIMPLE SEQUENCE REPEAT MARKERS. MODERN GENETICS JOURNAL (MGJ)[Internet]. 2015;9(4 (39)):517-523. Available from: https://sid.ir/paper/116694/en

IEEE: Copy

E. VAGHARI AZAR, H. HATAMI MALEKI, A. BASIRNIA, D. AHMADI, and R. DARVISHZADEH, “EVALUATION OF GENETIC VARIATION IN SOME IRANIAN ORIENTAL AND SEMI ORIENTAL TOBACCO GERMPLASMS BY USING SIMPLE SEQUENCE REPEAT MARKERS,” MODERN GENETICS JOURNAL (MGJ), vol. 9, no. 4 (39), pp. 517–523, 2015, [Online]. Available: https://sid.ir/paper/116694/en

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