EVALUATION OF ALLELIC VARIATION FOR LOW MOLECULAR WEIGHT GLUTENIN SUBUNITS USING DNA SPECIFIC MARKERS IN WHEAT LANDRACES

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BOZORGMEHR A.; AHMADI J.; SHAHINNIA F.; RAZAVI KH.; NAJAFIAN G.; LOHRASEBI T.

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Journal: MODERN GENETICS JOURNAL (MGJ) Year:2015 | Volume:9 | Issue:4 (39) Page(s): 439-450

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Title

EVALUATION OF ALLELIC VARIATION FOR LOW MOLECULAR WEIGHT GLUTENIN SUBUNITS USING DNA SPECIFIC MARKERS IN WHEAT LANDRACES

Author(s)

Keywords

ALLELE VARIATIONQ1

GLUTENINQ1

LMW-GSQ2

MARKERQ2

WHEATQ2

Abstract

This study was conducted to assess the low molecular weight GLUTENIN subunits allelic variation among 97 lines of bread and durum WHEAT landraces using 3 primer pairs for loci (Glu-A3, Glu-B3 and Glu-D3). Results showed significant variation among durum and bread WHEAT landraces. In general, five amplified band segments by primer Glu3-D.2, inclusive four alleles a, b, c and d among landraces were identified with the frequency of 0.013, 0.853, 0.026 and 0.054, respectively, and allele b was the most frequent. Four amplified band patterns by primer Glu3-3.1, inclusive 6 alleles a, b, c, d, e and f were identified with the frequency of 0.474, 0.474, 0.525, 0742, 0.742 and 0.164, respectively and allele d and e were the most frequent. Four amplified band patterns by primer Glu3-3.2 inclusive 10 alleles a, b, c, d, e, f, g, h, i and j were identified with the frequency of 0.010, 0.536, 0.010, 0.010, 0.010, 0.020, 0.659, 0.546, 0.010 and 0.010, respectively, and allele g was the most frequent. Using Nei’s genetic variation index (H), allelic variation for primers Glu3-D2, Glu3-3.1 and Glu3-3.2 was 0.314, 0.829 and 0.709, respectively. Identified variations in this study may be valuable as allelic diversity in bread and durum WHEAT breeding programs to improve the quality of their end products.

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APA: Copy

BOZORGMEHR, A., AHMADI, J., SHAHINNIA, F., RAZAVI, KH., NAJAFIAN, G., & LOHRASEBI, T.. (2015). EVALUATION OF ALLELIC VARIATION FOR LOW MOLECULAR WEIGHT GLUTENIN SUBUNITS USING DNA SPECIFIC MARKERS IN WHEAT LANDRACES. MODERN GENETICS JOURNAL (MGJ), 9(4 (39)), 439-450. SID. https://sid.ir/paper/116696/en

Vancouver: Copy

BOZORGMEHR A., AHMADI J., SHAHINNIA F., RAZAVI KH., NAJAFIAN G., LOHRASEBI T.. EVALUATION OF ALLELIC VARIATION FOR LOW MOLECULAR WEIGHT GLUTENIN SUBUNITS USING DNA SPECIFIC MARKERS IN WHEAT LANDRACES. MODERN GENETICS JOURNAL (MGJ)[Internet]. 2015;9(4 (39)):439-450. Available from: https://sid.ir/paper/116696/en

IEEE: Copy

A. BOZORGMEHR, J. AHMADI, F. SHAHINNIA, KH. RAZAVI, G. NAJAFIAN, and T. LOHRASEBI, “EVALUATION OF ALLELIC VARIATION FOR LOW MOLECULAR WEIGHT GLUTENIN SUBUNITS USING DNA SPECIFIC MARKERS IN WHEAT LANDRACES,” MODERN GENETICS JOURNAL (MGJ), vol. 9, no. 4 (39), pp. 439–450, 2015, [Online]. Available: https://sid.ir/paper/116696/en

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