ENZYMOLOGICAL CHARACTERISTICS OF PLASMA MEMBRANE PHOSPHATIDATE PHOSPHOHYDROLASE (PAP2) FROM RAT LIVER

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HEYDARIAN E.; HAGHIGHI B.

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Journal: IRANIAN JOURNAL OF SCIENCE AND TECHNOLOGY TRANSACTION A- SCIENCE Year:2008 | Volume:32 | Issue:A2 Page(s): 117-127

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Title

ENZYMOLOGICAL CHARACTERISTICS OF PLASMA MEMBRANE PHOSPHATIDATE PHOSPHOHYDROLASE (PAP2) FROM RAT LIVER

Author(s)

HEYDARIAN E. | HAGHIGHI B. | Issue Writer Certificate

Keywords

PHOSPHATIDATE PHOSPHOHYDROLASEQ3

PHOSPHATIDIC ACIDQ3

Abstract

PHOSPHATIDATE PHOSPHOHYDROLASE (PAP2b, fraction b) was purified from the plasma membrane of rat liver cells. The Km for the surface concentration of PHOSPHATIDIC ACID was 0.43 mol%. The subunit of the enzyme had an M.W. of 33.8 kDa using sodium dodecyl sulfate polyacrylamide gel electrophoresis. The native enzyme shows a molecular weight of 182 kDa in a gel filtration column packed with Sephacryl S300 in the presence of Triton X-100. The pH optima obtained for PAP2b were 5.5 and 7 in imidazole and Tris- HCl buffers, respectively. The membrane homogenate enzyme (PAP2) consumed the lamellar (La) phase of phosphatidate and was activated (approximately 3-fold) by Lubrol PX, CTAB and Tween 80 and inhibited by Zn2+ and Mn2+. The inhibition was concentration dependent. These cations affected PAP2b activity through the phase transition of phosphatidate from lamellar (La) to inverted hexagonal (HII) form. Guanidine hydrochloride and urea increased PAP2 activity (2-fold) up to 20mM concentrations by stabilizing the La phase. Optimum activity of purified PAP2b was obtained at 3% trehalose and 7% sucrose. The data suggested that the stability of the La form of phosphatidate by detergent micelles may take place through surface dilution processes.

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APA: Copy

HEYDARIAN, E., & HAGHIGHI, B.. (2008). ENZYMOLOGICAL CHARACTERISTICS OF PLASMA MEMBRANE PHOSPHATIDATE PHOSPHOHYDROLASE (PAP₂) FROM RAT LIVER. IRANIAN JOURNAL OF SCIENCE AND TECHNOLOGY TRANSACTION A- SCIENCE, 32(A2), 117-127. SID. https://sid.ir/paper/117960/en

Vancouver: Copy

HEYDARIAN E., HAGHIGHI B.. ENZYMOLOGICAL CHARACTERISTICS OF PLASMA MEMBRANE PHOSPHATIDATE PHOSPHOHYDROLASE (PAP₂) FROM RAT LIVER. IRANIAN JOURNAL OF SCIENCE AND TECHNOLOGY TRANSACTION A- SCIENCE[Internet]. 2008;32(A2):117-127. Available from: https://sid.ir/paper/117960/en

IEEE: Copy

E. HEYDARIAN, and B. HAGHIGHI, “ENZYMOLOGICAL CHARACTERISTICS OF PLASMA MEMBRANE PHOSPHATIDATE PHOSPHOHYDROLASE (PAP₂) FROM RAT LIVER,” IRANIAN JOURNAL OF SCIENCE AND TECHNOLOGY TRANSACTION A- SCIENCE, vol. 32, no. A2, pp. 117–127, 2008, [Online]. Available: https://sid.ir/paper/117960/en

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