DETECTION OF PAENIBACILLUS LARVAE LARVAE SPORES IN HONEY AND DISEASED LARVAE SAMPLES BY CULTURE AND PCR

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MOHARRAMI M.; MODIRROUSTA H.; MOEINFAR N.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Journal: ARCHIVES OF RAZI INSTITUTE Year:2011 | Volume:66 | Issue:2 Page(s): 101-107

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Title

DETECTION OF PAENIBACILLUS LARVAE LARVAE SPORES IN HONEY AND DISEASED LARVAE SAMPLES BY CULTURE AND PCR

Author(s)

MOHARRAMI M. | MODIRROUSTA H. | MOEINFAR N. | Issue Writer Certificate

Keywords

HONY BEEQ3

PAENIBACILLUS LARVAEQ3

AMERICAN FOULBROOD (AFB)Q3

BROOD DISEASEQ3

PCRQ3

Abstract

American foulbrood (AFB) is the most serious BROOD DISEASE of the honey bee. Traditional methods are reliable but rather slow simply because they are based on biochemical, morphological and physiological identification of cultivated isolates. The aim of this study is the detection of PAENIBACILLUS LARVAE larvae spores in honey and diseased larvae samples by culture and PCR. Therefore 54 samples of diseased larvae and 36 honey samples, were diluted with an equal volume of distilled water and centrifuged, then the pellet was used for bacterial culture, DNA extraction and PCR. PCR products were electrophoresed on 0.8 % agarose gel. Five of 54 (9.3 %) larvae samples and 5 of 36 (13.9 %) honey samples were positive for PAENIBACILLUS LARVAE larvae by culture and PCR. As a result, screening of honey and larvae samples by PCR method proved to be a reliable, fast and useful method on regional and national scale for monitoring, controlling, and using preventive measures before the occurrence of American foulbrood damages.

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APA: Copy

MOHARRAMI, M., MODIRROUSTA, H., & MOEINFAR, N.. (2011). DETECTION OF PAENIBACILLUS LARVAE LARVAE SPORES IN HONEY AND DISEASED LARVAE SAMPLES BY CULTURE AND PCR. ARCHIVES OF RAZI INSTITUTE, 66(2), 101-107. SID. https://sid.ir/paper/120094/en

Vancouver: Copy

MOHARRAMI M., MODIRROUSTA H., MOEINFAR N.. DETECTION OF PAENIBACILLUS LARVAE LARVAE SPORES IN HONEY AND DISEASED LARVAE SAMPLES BY CULTURE AND PCR. ARCHIVES OF RAZI INSTITUTE[Internet]. 2011;66(2):101-107. Available from: https://sid.ir/paper/120094/en

IEEE: Copy

M. MOHARRAMI, H. MODIRROUSTA, and N. MOEINFAR, “DETECTION OF PAENIBACILLUS LARVAE LARVAE SPORES IN HONEY AND DISEASED LARVAE SAMPLES BY CULTURE AND PCR,” ARCHIVES OF RAZI INSTITUTE, vol. 66, no. 2, pp. 101–107, 2011, [Online]. Available: https://sid.ir/paper/120094/en

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