COMPARATIVE STUDY OF THREE VACCINAL STRAINS OF CLOSTRIDIUM TETANI INCLUDING HARVARD 52, G5 AND 49205 FROM STANDPOINT OF SIX ESSENTIAL FACTORS TO EVALUATE THEIR TOXIGENESIS FOR USE IN TETANUS VACCINE PRODUCTION

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MOTEDAYEN M.H.; ABBASI E.; MADANI F.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: ARCHIVES OF RAZI INSTITUTE Year:2012 | Volume:67 | Issue:2 Page(s): 139-143

Download Full-Text

Persian Verion

View:

1,463

Download:

431

Cites:

Information Journal Paper

Title

COMPARATIVE STUDY OF THREE VACCINAL STRAINS OF CLOSTRIDIUM TETANI INCLUDING HARVARD 52, G5 AND 49205 FROM STANDPOINT OF SIX ESSENTIAL FACTORS TO EVALUATE THEIR TOXIGENESIS FOR USE IN TETANUS VACCINE PRODUCTION

Author(s)

MOTEDAYEN M.H. | ABBASI E. | MADANI F. | Issue Writer Certificate

Keywords

CLOSTRIDIUM TETANIQ3

HARVARD 52Q3

HARVARD G5Q3

HARVARD 49205Q3

TOXIGENESISQ3

Abstract

Tetanus is an important disease which created by tetanospasmin toxin of CLOSTRIDIUM TETANI. In this study we surveyed six important factors including LF, KF, MLD, pH, OD and total protein assay of HARVARD 52 (H52), G5 (HG5) and 49205 (H49205) strains of the bacterium to determine which of them were more suitable for use in vaccine production. The mentioned strains were seperatedly reactivated in thioglycolate medium, 2ml of this suspension was used to inoculate the Muller-Miller medium of each of sixteen 500 ml Erlenmeyer flasks (7,6 and 3 flasks for H52, HG5 and H49205 respectively), where the fermentation runs were performed. Over a period of seven days of experiment, several tests for evaluation of the six mentioned factors on samples of medium cultures were carried out. Results revealed that H52 strain had significantly lower values in LF and OD compared to strains HG5 & H49205 (P<0.001 and P<0.01 respectively) while its MLD and pH were better than other strains (P<0.05 and P<0.02 respectively). In conclusion, it seems that HG5 & H49205 strains have been greater toxin producer than H52 strain and as a result, we hope that with some complementary works, these two strains such as H52 strain, be used for routine tetanus vaccine production.

Cites

No record.

References

No record.

Cite

APA: Copy

MOTEDAYEN, M.H., ABBASI, E., & MADANI, F.. (2012). COMPARATIVE STUDY OF THREE VACCINAL STRAINS OF CLOSTRIDIUM TETANI INCLUDING HARVARD 52, G5 AND 49205 FROM STANDPOINT OF SIX ESSENTIAL FACTORS TO EVALUATE THEIR TOXIGENESIS FOR USE IN TETANUS VACCINE PRODUCTION. ARCHIVES OF RAZI INSTITUTE, 67(2), 139-143. SID. https://sid.ir/paper/120111/en

Vancouver: Copy

MOTEDAYEN M.H., ABBASI E., MADANI F.. COMPARATIVE STUDY OF THREE VACCINAL STRAINS OF CLOSTRIDIUM TETANI INCLUDING HARVARD 52, G5 AND 49205 FROM STANDPOINT OF SIX ESSENTIAL FACTORS TO EVALUATE THEIR TOXIGENESIS FOR USE IN TETANUS VACCINE PRODUCTION. ARCHIVES OF RAZI INSTITUTE[Internet]. 2012;67(2):139-143. Available from: https://sid.ir/paper/120111/en

IEEE: Copy

M.H. MOTEDAYEN, E. ABBASI, and F. MADANI, “COMPARATIVE STUDY OF THREE VACCINAL STRAINS OF CLOSTRIDIUM TETANI INCLUDING HARVARD 52, G5 AND 49205 FROM STANDPOINT OF SIX ESSENTIAL FACTORS TO EVALUATE THEIR TOXIGENESIS FOR USE IN TETANUS VACCINE PRODUCTION,” ARCHIVES OF RAZI INSTITUTE, vol. 67, no. 2, pp. 139–143, 2012, [Online]. Available: https://sid.ir/paper/120111/en

Related Journal Papers