PCR DETECTION OF ESCHERICHIA COLI 0157:H7 DIRECTED FROM SLAUGHTERED CATTLE IN SHIRAZ, IRAN

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TAHAMTAN Y.E.; SHEKARFOROUSH SEYED SHAHRAM; POURBAKHSH S.A.

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Journal: ARCHIVES OF RAZI INSTITUTE Year:2006 | Volume:61 | Issue:1 Page(s): 1-6

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Title

PCR DETECTION OF ESCHERICHIA COLI 0157:H7 DIRECTED FROM SLAUGHTERED CATTLE IN SHIRAZ, IRAN

Author(s)

TAHAMTAN Y.E. | SHEKARFOROUSH SEYED SHAHRAM | POURBAKHSH S.A. | Issue Writer Certificate

Keywords

ESCHERICHIA COLI 0157: H7Q4

STX2Q4

CATTLEQ4

PCRQ4

Abstract

Escherichia coli 0157:H7 lives in the intestines of healthy CATTLE, and can contaminate meat during slaughtering practices. Detection of the low infectious dosage of bacterium requires a sensitive method. We developed polymerase chain reaction (PCR) assays to detect the gene Stx2 irrespective of the bacterial serotype. In this study, the detection limit of the PCR protocol in detecting StX2 in E. coli 0157:H7 without prior enrichment was 103 CFU/ml, and with 4 h prior enrichment in modified tryptic soy broth was 102 CFU/ml. During a period of 7 months (December 2004 to June 2005), 154 slaughtered CATTLE at Shiraz slaughterhouse, were randomly selected and examined for surface carriage of E. coli 0 157:H7 via detection the Stx2 gene of verotoxigenic E. coli by PCR technique. E. coli 0157:H7 was found in 14 of 145 (9.65%) feedlot CATTLE and in 1 of 9 (11.1%) dairy cull cows. This is the first report of the presence of E. coli 0 157:H7 in CATTLE from Iran.

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APA: Copy

TAHAMTAN, Y.E., SHEKARFOROUSH, SEYED SHAHRAM, & POURBAKHSH, S.A.. (2006). PCR DETECTION OF ESCHERICHIA COLI 0157:H7 DIRECTED FROM SLAUGHTERED CATTLE IN SHIRAZ, IRAN. ARCHIVES OF RAZI INSTITUTE, 61(1), 1-6. SID. https://sid.ir/paper/120121/en

Vancouver: Copy

TAHAMTAN Y.E., SHEKARFOROUSH SEYED SHAHRAM, POURBAKHSH S.A.. PCR DETECTION OF ESCHERICHIA COLI 0157:H7 DIRECTED FROM SLAUGHTERED CATTLE IN SHIRAZ, IRAN. ARCHIVES OF RAZI INSTITUTE[Internet]. 2006;61(1):1-6. Available from: https://sid.ir/paper/120121/en

IEEE: Copy

Y.E. TAHAMTAN, SEYED SHAHRAM SHEKARFOROUSH, and S.A. POURBAKHSH, “PCR DETECTION OF ESCHERICHIA COLI 0157:H7 DIRECTED FROM SLAUGHTERED CATTLE IN SHIRAZ, IRAN,” ARCHIVES OF RAZI INSTITUTE, vol. 61, no. 1, pp. 1–6, 2006, [Online]. Available: https://sid.ir/paper/120121/en

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