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Information Journal Paper

Title

MICROPROPAGATION OF MATURE AND SEEDLING SPECIMEN OF EUCALYPTUS GLOBULUS

Pages

  139-149

Abstract

 EUCALYPTUS GLOBULUS is one of the most important and fast growing species used in afforestation for wood and paper industries, essence and honey production. Due to the graft process incompatibility, as well as hard proliferation of roots in adult tissues, in this study the plant reproduction has been carried out through MICROPROPAGATION methods Vegetative lateral buds were collected from selected adult and SEEDLING in northern forests in different seasons and disinfected with seveal solutions of mercury chloride and sodium hypochlorite in different concentrations and times intervals depending on season and plant genotype. Then, the effects of five hormonal treatments, including different concentrations of cytokinins and auxines (0 to 1 mgl-1) on developing branches from MICROPROPAGATION and SEEDLING buds were studied in three replicated experiments with a statistical variance analysis based on a completely randomized design. Different characteristics such as shoot length, proliferation and greenish coefficient of shoot were measured. The most appropriate method of sterilization was immersion of buds in solution of 70% ethanol for 30 seconds and then in 0.5 % mercury chloride solution for 10 minutes in spring for MATURE specimen buds and 0.1 % mercuric chloride solution for 6 minutes in spring for SEEDLING buds. The best proliferation of shoots from MATURE buds was achieved in MS medium (half strength Nitrate) containing 1 mgl-1 Zeatin and 0.2 mgl-1 IAA and for shoots from SEEDLING buds was occurred at the same medium containing 0.5 mgl-1 BAP, 0.2 mgl-1 Kin, 0.1 mgl-1 GA3 and 0.01 mgl-1 IBA. Root germination in shoots with a length of 1.5 cm from the MATURE and SEEDLING sources occurred in 1.4 concentration medium of MS macro elements and 0.5 mgl-1 of combined auxin hormones of NAA and IBA. After gradual adaptation of plantlets under greenhouse conditions, they were transferred and established successfully in soil.

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    Cite

    APA: Copy

    EMAM, MITRA, & ASAREH, M.H.. (2010). MICROPROPAGATION OF MATURE AND SEEDLING SPECIMEN OF EUCALYPTUS GLOBULUS. IRANIAN JOURNAL OF FOREST, 2(2), 139-149. SID. https://sid.ir/paper/123035/en

    Vancouver: Copy

    EMAM MITRA, ASAREH M.H.. MICROPROPAGATION OF MATURE AND SEEDLING SPECIMEN OF EUCALYPTUS GLOBULUS. IRANIAN JOURNAL OF FOREST[Internet]. 2010;2(2):139-149. Available from: https://sid.ir/paper/123035/en

    IEEE: Copy

    MITRA EMAM, and M.H. ASAREH, “MICROPROPAGATION OF MATURE AND SEEDLING SPECIMEN OF EUCALYPTUS GLOBULUS,” IRANIAN JOURNAL OF FOREST, vol. 2, no. 2, pp. 139–149, 2010, [Online]. Available: https://sid.ir/paper/123035/en

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