BORDETELLA PERTUSSISFIMBRIAE (FIM2 AND FIM3) EXPRESSION EVALUATION IN DIFFERENT STAGES OF CULTIVATION TO PRODUCE VACCINE

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LATIFI TAYEBE; NOOFELI MOJTABA; Jabalameli Leila

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Journal: Jundishapur Scientific Medical Journal Year:2017 | Volume:16 | Issue:3 Page(s): 345-354

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Title

BORDETELLA PERTUSSISFIMBRIAE (FIM2 AND FIM3) EXPRESSION EVALUATION IN DIFFERENT STAGES OF CULTIVATION TO PRODUCE VACCINE

Author(s)

LATIFI TAYEBE | NOOFELI MOJTABA | Jabalameli Leila | Issue Writer Certificate

Keywords

BORDETELLA PERTUSSISQ2

VACCINEQ1

ELISAQ2

FIMBRIAEQ3

FIM2Q1

FIM3Q1

Abstract

Background and Objective: FIMBRIAE (Fim2 and FIM3) is one of the most important virulence factors in BORDETELLA PERTUSSIS and it causes the adhesion of bacteria to the upper respiratory tract cells and that enhances immune response. The level of antibody production against FIMBRIAE is directly related to the level of protection. FIMBRIAE is one of the main components in both wholecell and acellular pertussis VACCINEs. According to WHO standard, in the whole-cell VACCINE, presence of FIMBRIAE must be confirmed by two techniques including ELISA and slide agglutination.Subjects and Methods: In this research, two strains of B. pertussis 509 (with FIM2) and 134 (with FIM3) were tested by culturing in B2 and presentation of FIMBRIAE was assessed at different stages of cultivation. In this study indirect ELISA and slide agglutination assays were used to determine and evaluate the presence and quantity of the FIMBRIAE during cultivation period. After confirming the presence of the FIMBRIAE, two strains (134 and 509) were cultured in B2 medium.Results: According to the FIMBRIAE expression, the best time for stopping and harvesting the cultivation were determined.Conclusion: The results have shown that the high level of FIM2 and FIM3 expression and the best time for harvesting and stopping the culture is in the log phase. Although, the level of expression in two strains were dissimilar in this phase, but eventually the most expression level is in the log phase.

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APA: Copy

LATIFI, TAYEBE, NOOFELI, MOJTABA, & Jabalameli, Leila. (2017). BORDETELLA PERTUSSISFIMBRIAE (FIM2 AND FIM3) EXPRESSION EVALUATION IN DIFFERENT STAGES OF CULTIVATION TO PRODUCE VACCINE. JUNDISHAPUR SCIENTIFIC MEDICAL JOURNAL, 16(3 ), 345-354. SID. https://sid.ir/paper/12725/en

Vancouver: Copy

LATIFI TAYEBE, NOOFELI MOJTABA, Jabalameli Leila. BORDETELLA PERTUSSISFIMBRIAE (FIM2 AND FIM3) EXPRESSION EVALUATION IN DIFFERENT STAGES OF CULTIVATION TO PRODUCE VACCINE. JUNDISHAPUR SCIENTIFIC MEDICAL JOURNAL[Internet]. 2017;16(3 ):345-354. Available from: https://sid.ir/paper/12725/en

IEEE: Copy

TAYEBE LATIFI, MOJTABA NOOFELI, and Leila Jabalameli, “BORDETELLA PERTUSSISFIMBRIAE (FIM2 AND FIM3) EXPRESSION EVALUATION IN DIFFERENT STAGES OF CULTIVATION TO PRODUCE VACCINE,” JUNDISHAPUR SCIENTIFIC MEDICAL JOURNAL, vol. 16, no. 3 , pp. 345–354, 2017, [Online]. Available: https://sid.ir/paper/12725/en

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