ISOLATION OF B-1 SUBTYPE B LYMPHOCYTES FROM HUMAN CORD BLOOD

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SANCHOOLI J.; MOSAFA N.; SHAHRAKI VAHED A.; FARJAH GH.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Pars Journal of Medical Sciences Year:2013 | Volume:11 | Issue:1 Page(s): 33-40

Download Full-Text

Persian Verion

View:

1,697

Download:

Cites:

Information Journal Paper

Title

ISOLATION OF B-1 SUBTYPE B LYMPHOCYTES FROM HUMAN CORD BLOOD

Author(s)

SANCHOOLI J. | MOSAFA N. | SHAHRAKI VAHED A. | FARJAH GH. | Issue Writer Certificate

Keywords

B LYMPHOCYTESQ3

BLOODQ3

MACROPHAGEQ2

Abstract

Introduction: B1 lymphocytes by having a large supply of CD5 as a marker have the capability of cell differentiation and distinguishing them into cells similar to MACROPHAGEs B1 Cell. The aim of the study is to determine the possibility of separation and purification of this unique category from other cellular organizations of cord BLOOD lymphocytes.Materials and Methods: 14 samples of the umbilical cord BLOOD from normal delivery or elective cesarean were used. Dilutions of cells to purified circulating Leukocytes by the use of hydroxyl ethyl starch or 3% gelatin have been made and WBC was separated from RBC. In order to separate adhesive or non-adhesve cells, cell culture was done. By gradient ficoll and centrifuge, lymphocyte cells of the umbilical cord from the remaining leukocytes and mononuclear cells were purified and they were extracted from B LYMPHOCYTES by nylon wool columns. And rosette test was used for testing the extracted cells.Results: In spite of lack of difference between cellular counts in the two BLOOD dilution materials, the amount of cellular survival showed to be lower than 87% in hydroxyl ethyl starch method while in the dilution method with gelatin 3%, the cellular life was about 90-95%. The results of Rosette test showed that dilution amount of B1 cells by the above method was about 99%.Conclusion: This process reveals that most of B cells extracted from cord BLOOD are the of B1 cell type and the best and most suitable technique is gelatin 3% procedure with regard to the number and amount of cellular’s life.

Cites

IGM+CD5+ CELLS IN CANCEROUS MURINE MODEL USING BCL1 CELL LINE

References

No record.

Cite

APA: Copy

SANCHOOLI, J., MOSAFA, N., SHAHRAKI VAHED, A., & FARJAH, GH.. (2013). ISOLATION OF B-1 SUBTYPE B LYMPHOCYTES FROM HUMAN CORD BLOOD. PARS JOURNAL OF MEDICAL SCIENCES (JAHROM MEDICAL JOURNAL), 11(1), 33-40. SID. https://sid.ir/paper/130867/en

Vancouver: Copy

SANCHOOLI J., MOSAFA N., SHAHRAKI VAHED A., FARJAH GH.. ISOLATION OF B-1 SUBTYPE B LYMPHOCYTES FROM HUMAN CORD BLOOD. PARS JOURNAL OF MEDICAL SCIENCES (JAHROM MEDICAL JOURNAL)[Internet]. 2013;11(1):33-40. Available from: https://sid.ir/paper/130867/en

IEEE: Copy

J. SANCHOOLI, N. MOSAFA, A. SHAHRAKI VAHED, and GH. FARJAH, “ISOLATION OF B-1 SUBTYPE B LYMPHOCYTES FROM HUMAN CORD BLOOD,” PARS JOURNAL OF MEDICAL SCIENCES (JAHROM MEDICAL JOURNAL), vol. 11, no. 1, pp. 33–40, 2013, [Online]. Available: https://sid.ir/paper/130867/en

Related Journal Papers