MOLECULAR DETECTION OF BACTERIAL PATHOGENS INVOLVED IN URINARY TRACT INFECTION

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SEDIGHIAN HOUMAN; POURMAND MOHAMMAD REZA

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Journal: IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY Year:2008 | Volume:1 | Issue:4 Page(s): 41-45

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Information Journal Paper

Title

MOLECULAR DETECTION OF BACTERIAL PATHOGENS INVOLVED IN URINARY TRACT INFECTION

Author(s)

SEDIGHIAN HOUMAN | POURMAND MOHAMMAD REZA | Issue Writer Certificate

Keywords

UTIQ3

UNIVERSAL PRIMERSQ4

PCRQ3

RFLPQ4

Abstract

Background and Objectives: Urinary tract infection (UTI) is one of the most common infections and a major cause of patient morbidity world wide. The gold standard for detection of bacterial pathogens in UTI is culture of urine specimens. In order to facilitate identification of bacterial pathogens in clinical urine specimens we designed a molecular method to detect bacterial pathogen in UTI.Material and Methods: The genomic DNA of standard bacterial pathogens involved in UTI was extracted. Based on the 16S rDNA, the UNIVERSAL PRIMERS were designed and the amplification of the fragments was carried out. The enzymatic digestions were performed by two restriction enzymes (HaeIII and AluI) to produce a specific pattern for bacteria as follow; E. coli, K. pneumoniae, S. saprophyticus, S. aureus, P. aeruginosa and P. mirabilis.Results: Although the size of the PCR products were not quite changed in different species, but the pattern of digestion is exclusive. The polyacrylamide gel electrophoresis showed a significant differentiations bands.

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APA: Copy

SEDIGHIAN, HOUMAN, & POURMAND, MOHAMMAD REZA. (2008). MOLECULAR DETECTION OF BACTERIAL PATHOGENS INVOLVED IN URINARY TRACT INFECTION. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, 1(4), 41-45. SID. https://sid.ir/paper/132529/en

Vancouver: Copy

SEDIGHIAN HOUMAN, POURMAND MOHAMMAD REZA. MOLECULAR DETECTION OF BACTERIAL PATHOGENS INVOLVED IN URINARY TRACT INFECTION. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY[Internet]. 2008;1(4):41-45. Available from: https://sid.ir/paper/132529/en

IEEE: Copy

HOUMAN SEDIGHIAN, and MOHAMMAD REZA POURMAND, “MOLECULAR DETECTION OF BACTERIAL PATHOGENS INVOLVED IN URINARY TRACT INFECTION,” IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, vol. 1, no. 4, pp. 41–45, 2008, [Online]. Available: https://sid.ir/paper/132529/en

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