Isolation of Free-living amoeba and Molecular Characterization of Acanthamoeba From Stagnant Water, Kashan, Iran

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Golestani Mohammad Hossein; RASTI SIMA; HOOSHYAR HOSSEIN; DELAVARI MAHDI; MOUSAVI SEYED GHOLAM ABBAS; IRANSHAHI LEILA

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY Year:2018 | Volume:12 | Issue:2 Page(s): 125-132

Download Full-Text

Persian Verion

View:

547

Download:

Cites:

Information Journal Paper

Title

Isolation of Free-living amoeba and Molecular Characterization of Acanthamoeba From Stagnant Water, Kashan, Iran

Author(s)

Keywords

Free-living amoebaQ1

AcanthamoebaQ1

Stagnant WaterQ1

GenotypeQ1

Abstract

Background and Aims: Acanthamoeba, the causative agent of granulomatous amebic encephalitis )GAE), is among the most prevalent Free-living amoebas (FLA) existing in water, soil and dust. This study was conducted to determine FLA and identify Acanthamoeba Genotypes isolated from Stagnant Water in Kashan, Iran. Materials and Methods: In this descriptive study, 138 Stagnant Water samples were collected from Kashan mosques and public parks. The samples were filtered (0. 45μ m) and cultured onto non-nutrient agar for the presence of FLA. Acanthamoeba spp. was identified by polymerase chain reaction (PCR) using specific primers, which amplified a 490 bp fragment. Among ten sequenced isolates of Acanthamoeba, different Genotypes were determined by sequence analysis. The parameters such as pH, temperature, sampling season and related results were recorded and analyzed using SPSS16. Results: The rate of FLA was 88. 4 %, 59. 4% of which were confirmed as Acanthamoeba spp. using PCR method. The rate of Acanthamoeba T4 and T2 Genotypes were 80% and 20%, respectively. There was a significant relation between FLA rate and sampling season (P= 0. 01). The highest rate of FLA and Acanthamoeba was observed at pH 7. There was no significant relationship between FLA and Acanthamoeba spp. with pH and temperature. Conclusions: The rate of FLA and Acanthamoeba in Stagnant Water were high in Kashan. The dominant Acanthamoeba Genotype (T4) is pathogen. Due to serious amoeba-induced complications, hygienic education is recommended to increase the public awareness on transmission and health/preventive measurements.

Cites

No record.

References

No record.

Cite

APA: Copy

Golestani, Mohammad Hossein, RASTI, SIMA, HOOSHYAR, HOSSEIN, DELAVARI, MAHDI, MOUSAVI, SEYED GHOLAM ABBAS, & IRANSHAHI, LEILA. (2018). Isolation of Free-living amoeba and Molecular Characterization of Acanthamoeba From Stagnant Water, Kashan, Iran. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, 12(2 ), 125-132. SID. https://sid.ir/paper/132546/en

Vancouver: Copy

Golestani Mohammad Hossein, RASTI SIMA, HOOSHYAR HOSSEIN, DELAVARI MAHDI, MOUSAVI SEYED GHOLAM ABBAS, IRANSHAHI LEILA. Isolation of Free-living amoeba and Molecular Characterization of Acanthamoeba From Stagnant Water, Kashan, Iran. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY[Internet]. 2018;12(2 ):125-132. Available from: https://sid.ir/paper/132546/en

IEEE: Copy

Mohammad Hossein Golestani, SIMA RASTI, HOSSEIN HOOSHYAR, MAHDI DELAVARI, SEYED GHOLAM ABBAS MOUSAVI, and LEILA IRANSHAHI, “Isolation of Free-living amoeba and Molecular Characterization of Acanthamoeba From Stagnant Water, Kashan, Iran,” IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, vol. 12, no. 2 , pp. 125–132, 2018, [Online]. Available: https://sid.ir/paper/132546/en

Related Journal Papers