GENOTYPING OF HELICOBACTER PYLORI STRAINS ISOLATED FROM PATIENTS WITH NUD, DU, GU AND GC BY RAPD-PCR

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GHASEMI AMIR; SHIRAZI M.H.; POURMAND M.R.; ZAEIMI YAZDI J.; SADEGHIFARD NOUR KHODA; BAGHERZADEH JALILVAND S.; AGHA AMIRI SOULMAZ

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Journal: IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY Year:2007 | Volume:1 | Issue:2 Page(s): 21-25

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Title

GENOTYPING OF HELICOBACTER PYLORI STRAINS ISOLATED FROM PATIENTS WITH NUD, DU, GU AND GC BY RAPD-PCR

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Keywords

HELICOBACTER PYLORIQ3

RAPD-PCRQ4

GENOTYPINGQ4

Abstract

Background and Objectives: HELICOBACTER PYLORI is a genetically diverse gastric pathogen that chronically infects billions of people worldwide, typically beginning in infancy and lasting for decades. It is a major cause of peptic ulcers and it is an early risk factor for gastric cancer which is the most frequently lethal malignancy globally. This project was designed to genotype H. pylori isolates isolated from patients with NUD, DU, GU and GC by the polymerase chain reaction (PCR)-based on Randomly Amplified Polymorphic DNA (RAPD) fingerprinting technique.Material and Methods: Eighty patients admitted to the gastroenterology unit at Sharyati hospital in Iran were included in this study. Gastric biopsy specimens were inoculated onto selective medium then were cultured for 3 to 5 days at 37°C under microaerobic conditions. Genomic DNA was extracted using a commercially available Qiagen kit. RAPD-PCR was used to genotype isolates.Results: Six different RAPD patterns (A-F) were seen in more than one isolate which were as follow; pattern A: 9 (16.98%), B: 6 (11.33%), C: 5 (9.43%), D: 3 (5.66%), E: 2 (3.77%) and F: 2 (3.77%). Twenty six (49.06%) of 53 isolates showed a unique RAPD pattern that were not similar to each other. A significant relationship was not seen between a single RAPD pattern and a gastric disorder (P>0.05). Conclusion: The results of this study suggest a high level of DNA sequence diversity among H. pylori isolates and it is better to use sequencing method for surveying of HELICOBACTER PYLORI genome rather than RAPD-PCR.

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APA: Copy

GHASEMI, AMIR, SHIRAZI, M.H., POURMAND, M.R., ZAEIMI YAZDI, J., SADEGHIFARD, NOUR KHODA, BAGHERZADEH JALILVAND, S., & AGHA AMIRI, SOULMAZ. (2007). GENOTYPING OF HELICOBACTER PYLORI STRAINS ISOLATED FROM PATIENTS WITH NUD, DU, GU AND GC BY RAPD-PCR. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, 1(2), 21-25. SID. https://sid.ir/paper/132558/en

Vancouver: Copy

GHASEMI AMIR, SHIRAZI M.H., POURMAND M.R., ZAEIMI YAZDI J., SADEGHIFARD NOUR KHODA, BAGHERZADEH JALILVAND S., AGHA AMIRI SOULMAZ. GENOTYPING OF HELICOBACTER PYLORI STRAINS ISOLATED FROM PATIENTS WITH NUD, DU, GU AND GC BY RAPD-PCR. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY[Internet]. 2007;1(2):21-25. Available from: https://sid.ir/paper/132558/en

IEEE: Copy

AMIR GHASEMI, M.H. SHIRAZI, M.R. POURMAND, J. ZAEIMI YAZDI, NOUR KHODA SADEGHIFARD, S. BAGHERZADEH JALILVAND, and SOULMAZ AGHA AMIRI, “GENOTYPING OF HELICOBACTER PYLORI STRAINS ISOLATED FROM PATIENTS WITH NUD, DU, GU AND GC BY RAPD-PCR,” IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, vol. 1, no. 2, pp. 21–25, 2007, [Online]. Available: https://sid.ir/paper/132558/en

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