ISOLATION AND IDENTIFICATION OF LACTOBACILLUS BACTERIA WITH PROBIOTIC POTENTIAL FROM TRADITIONAL DAIRY IN KERMAN

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

ROKHTABNAK NAJME; KHALEGHI MOUJ; SASAN HOSSEIN ALI

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY Year:2016 | Volume:10 | Issue:1 Page(s): 24-34

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

1,629

Download:

Cites:

Information Journal Paper

Title

ISOLATION AND IDENTIFICATION OF LACTOBACILLUS BACTERIA WITH PROBIOTIC POTENTIAL FROM TRADITIONAL DAIRY IN KERMAN

Author(s)

ROKHTABNAK NAJME | KHALEGHI MOUJ | SASAN HOSSEIN ALI | Issue Writer Certificate

Keywords

LACTOBACILLUSQ2

PROBIOTICQ2

ANTIMICROBIALQ2

16S RRNA GENEQ2

Abstract

Background and Aim: As lactobacilli possess an antagonistic growth property, therefore they may be used as bio protective agents for infection control .The aim of this study was to investigate the antagonistic effect of lactobacilli isolated from local dairy.Materials and Methods: In this study 26 species of Lactobacilli were isolated from milk, homemade yoghurt and cheese .Each isolated bacteria was tested for its tolerance to acidic environments) at pH, 0.2, 0.3 and 0.4 (and bile salt concentrations (0.3,0.5 and 1% w/v) .Also, isolates were further tested for their ANTIMICROBIAL activity against common pathogenic bacteria such as Enterococcus feacalis, Listeria monocytogenes, Staphylococcus aureus PTCC 1112, Escherichia coli PTCC 1330 and Bacillus cereus PTCC 1015 by using the agar well method. Then the strains that are not only resistant to acidic conditions and bile salts but also have ANTIMICROBIAL activity, were identified by 16S RRNA GENE sequencing.Results: In this study, most of isolated Lactobacilli were found to inhibit growth of pathogens (96.15%). 61.5% and 84.62% of isolated lactobacilli tolerated to pH 2 and 0.3% bile salt. Only two strains, 53C and 22CL, were more resistant to pH 2 and high concentration of bile salt. The Phylogenetic analysis based on 16S rRNA sequencing revealed that two strains (53C and 22CL) belong to species of LACTOBACILLUS casei.Conclusions: According the results the two isolated resistant strains can be employed as PROBIOTIC starter in PROBIOTIC dairy products preparation.

Cites

No record.

References

No record.

Cite

APA: Copy

ROKHTABNAK, NAJME, KHALEGHI, MOUJ, & SASAN, HOSSEIN ALI. (2016). ISOLATION AND IDENTIFICATION OF LACTOBACILLUS BACTERIA WITH PROBIOTIC POTENTIAL FROM TRADITIONAL DAIRY IN KERMAN. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, 10(1), 24-34. SID. https://sid.ir/paper/132607/en

Vancouver: Copy

ROKHTABNAK NAJME, KHALEGHI MOUJ, SASAN HOSSEIN ALI. ISOLATION AND IDENTIFICATION OF LACTOBACILLUS BACTERIA WITH PROBIOTIC POTENTIAL FROM TRADITIONAL DAIRY IN KERMAN. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY[Internet]. 2016;10(1):24-34. Available from: https://sid.ir/paper/132607/en

IEEE: Copy

NAJME ROKHTABNAK, MOUJ KHALEGHI, and HOSSEIN ALI SASAN, “ISOLATION AND IDENTIFICATION OF LACTOBACILLUS BACTERIA WITH PROBIOTIC POTENTIAL FROM TRADITIONAL DAIRY IN KERMAN,” IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, vol. 10, no. 1, pp. 24–34, 2016, [Online]. Available: https://sid.ir/paper/132607/en

Related Journal Papers