CLONING AND SEQUENCING OF SACOL A NOVEL GENE FROM STAPHYLOCOCCUS AUREUS

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MENBARI SH.; POURMAND MOHAMMAD REZA; SHIRAZI M.H.; MARDANI NADIA

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Information Journal Paper

Title

CLONING AND SEQUENCING OF SACOL A NOVEL GENE FROM STAPHYLOCOCCUS AUREUS

Author(s)

MENBARI SH. | POURMAND MOHAMMAD REZA | SHIRAZI M.H. | MARDANI NADIA | Issue Writer Certificate

Keywords

STAPHYLOCOCCUS AUREUSQ2

SACOLQ4

CLONINGQ3

Abstract

Background & Objectives: Natural staphylococcal infections and vaccines based whole bacteria lead to poor antibody responses, but recent research reveals that specific antibodies based on recombinant staphylococcal antigens are much more protective. SACOL is a novel antigen that its structural and immunological traits poorly characterized. This research aimed to clone of SACOL, a novel gene from STAPHYLOCOCCUS AUREUS.Material and Methods: The specific primers with suitable restriction sites were designed and SACOL amplified by PCR. The SACOL and plasmid were produced as sticky ends by restriction enzymes NdeI and XhoI. To amplify the recombinant plasmid the pET21sacol transferred into competent cell E.coliTOP10. The recombinant plasmid harvested from the host and analyzed by restriction enzymes and sequencing. Finally, SACOL gene analyzed by bioinformatics tools.Results: The SACOL gene has 723bp which amplified, cloned and sequenced successfully. SACOL is highly conserved in STAPHYLOCOCCUS AUREUS strains. Moreover, software analysis shows that SACOL encodes a protein with 32KDa molecular weight (267 amino acids) which has similarity with C51 peptidase in N-terminal with one alpha helix and 14 beta sheets.Conclusion: the SACOL gene is conserved in majority of STAPHYLOCOCCUS AUREUS strains and may exist and express in most of staphylococcal infections. The role and regulation of the gene is thus of great interest.

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APA: Copy

MENBARI, SH., POURMAND, MOHAMMAD REZA, SHIRAZI, M.H., & MARDANI, NADIA. (2008). CLONING AND SEQUENCING OF SACOL A NOVEL GENE FROM STAPHYLOCOCCUS AUREUS. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, 2(1), 9-14. SID. https://sid.ir/paper/132667/en

Vancouver: Copy

MENBARI SH., POURMAND MOHAMMAD REZA, SHIRAZI M.H., MARDANI NADIA. CLONING AND SEQUENCING OF SACOL A NOVEL GENE FROM STAPHYLOCOCCUS AUREUS. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY[Internet]. 2008;2(1):9-14. Available from: https://sid.ir/paper/132667/en

IEEE: Copy

SH. MENBARI, MOHAMMAD REZA POURMAND, M.H. SHIRAZI, and NADIA MARDANI, “CLONING AND SEQUENCING OF SACOL A NOVEL GENE FROM STAPHYLOCOCCUS AUREUS,” IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, vol. 2, no. 1, pp. 9–14, 2008, [Online]. Available: https://sid.ir/paper/132667/en

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