HETEROLOGOUS EXPRESSION OF THE SECALE CEREAL THAUMATINLIKE PROTEIN IN TRANSGENIC CANOLA PLANTS ENHANCES RESISTANCE TO STEM ROT DISEASE

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ZAMANI AKRAM; MOTALLEBI MOSTAFA; JONOUBI PARISA; GHAFARIAN NIA NAYERE SADAT; ZAMANI MOHAMMAD REZA

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Journal: Iranian Journal of Biotechnology Year:2012 | Volume:10 | Issue:2 Page(s): 87-95

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Title

HETEROLOGOUS EXPRESSION OF THE SECALE CEREAL THAUMATINLIKE PROTEIN IN TRANSGENIC CANOLA PLANTS ENHANCES RESISTANCE TO STEM ROT DISEASE

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Keywords

CANOLAQ2

SCLEROTINIA SCLEROTIORUMQ2

SECALE CEREALQ2

THAUMATIN-LIKE PROTEINSQ2

TRANSGENIC PLANTQ3

Abstract

CANOLA (Brassica napus L.) is an important oilseed crop. A serious problem in cultivation of this crop and yield loss, are due to fungal disease stem rot caused by SCLEROTINIA SCLEROTIORUM. The pathogenesis-related (PR) proteins have the potential for enhancing resistance against fungal pathogen. THAUMATIN-LIKE PROTEINS (TLPs) have been shown to have antifungal activity on various fungal pathogens. In this study, the tlp gene isolated from cereal rye (Secale cereal L.) was introduced into CANOLA plants. The amplified DNA fragment (about 500 bp) was analyzed and confirmed by restriction pattern and cloned into pUC19 and designated as pUCNG1. Comparison of the cloned fragment with the DNA sequence indicated that this gene contains no intron. The tlp gene was predicted to encode a protein of 173 amino acids with an estimated molecular mass of 17.7 kDa. The deduced amino acid sequence of TLP showed a significant sequence identity with TLP from S. cereal and other plants. We used a transgenic over-expression approach in order to investigate antifungal activity of expressed TLP on SCLEROTINIA SCLEROTIORUM. TLP was overexpressed under the CaMV35S constitutive promoter in (Brassica napus, R line Hyola 308). Transformation of cotyledonary petioles was achieved via Agrobacterium tumefaciens LBA4404. The insertion of transgene was verified by the polymerase chain reaction (PCR) and genomic DNA dot blotting. Antifungal activity was detected in transgenic CANOLA lines using detached leaf assay. The size of lesions induced by S. sclerotiorum in the leaves of transgenic CANOLA was significantly retarded when compared to that detected in non-transgenic plants.

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APA: Copy

ZAMANI, AKRAM, MOTALLEBI, MOSTAFA, JONOUBI, PARISA, GHAFARIAN NIA, NAYERE SADAT, & ZAMANI, MOHAMMAD REZA. (2012). HETEROLOGOUS EXPRESSION OF THE SECALE CEREAL THAUMATINLIKE PROTEIN IN TRANSGENIC CANOLA PLANTS ENHANCES RESISTANCE TO STEM ROT DISEASE. IRANIAN JOURNAL OF BIOTECHNOLOGY, 10(2), 87-95. SID. https://sid.ir/paper/139223/en

Vancouver: Copy

ZAMANI AKRAM, MOTALLEBI MOSTAFA, JONOUBI PARISA, GHAFARIAN NIA NAYERE SADAT, ZAMANI MOHAMMAD REZA. HETEROLOGOUS EXPRESSION OF THE SECALE CEREAL THAUMATINLIKE PROTEIN IN TRANSGENIC CANOLA PLANTS ENHANCES RESISTANCE TO STEM ROT DISEASE. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2012;10(2):87-95. Available from: https://sid.ir/paper/139223/en

IEEE: Copy

AKRAM ZAMANI, MOSTAFA MOTALLEBI, PARISA JONOUBI, NAYERE SADAT GHAFARIAN NIA, and MOHAMMAD REZA ZAMANI, “HETEROLOGOUS EXPRESSION OF THE SECALE CEREAL THAUMATINLIKE PROTEIN IN TRANSGENIC CANOLA PLANTS ENHANCES RESISTANCE TO STEM ROT DISEASE,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 10, no. 2, pp. 87–95, 2012, [Online]. Available: https://sid.ir/paper/139223/en

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