PURIFICATION AND CHARACTERIZATION OF A THERMOSTABLE NEUTROPHILIC METALLOPROTEASE FROM PSEUDOMONAS SP. DR89

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ASOODEH AHMAD; MOHAMMADIAN MUSAABADI HOSSEIN

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Journal: Iranian Journal of Biotechnology Year:2012 | Volume:10 | Issue:2 Page(s): 120-128

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Title

PURIFICATION AND CHARACTERIZATION OF A THERMOSTABLE NEUTROPHILIC METALLOPROTEASE FROM PSEUDOMONAS SP. DR89

Author(s)

ASOODEH AHMAD | MOHAMMADIAN MUSAABADI HOSSEIN | Issue Writer Certificate

Keywords

NEUTROPHILIC PROTEASEQ2

PHYLOGENETIC TREEQ2

THERMOSTABILITYQ3

PURIFICATIONQ3

CHARACTERIZATIONQ2

Abstract

A novel neutrophilic metalloprotease was isolated from Pseudomonassp. DR89 isolate which was identified in a mineral spring in Iran. The enzyme was purified from the isolate to 21-folds in a three-step procedure involving ammonium sulfate precipitation, Q-Sepharose ionic exchange and Sephadex G-100 gel filtration chromatography. Resuts showed that the enzyme was active at high temperatures and in a wide-range pH of 5-11 with the optimum of 8.0. The zymogram and sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis revealed the presence of one protease with a molecular weight of 74 kDa. The enzyme activity was decreased by Zn2+, Mn2+, H2O2 and cetyl trimethylammonium bromide (CTAB), whereas its activity was increased by Ca2+, Mg2+, Cu2+ and dimethyl sulfoxide (DMSO). Na+, phenylmethyl sulfonylfluoride (PMSF), b-mercaptoethanol, sodium dodecyl sulfate (SDS), and Triton X-100 did not show a considerable effect on its activity. Casein was a better substrate than bovin serum albumin (BSA) and gelatin for this enzyme. The kinetic parameters (Km and Vmax) of the purified protease towards caseinolytic activity were also determined. These properties of the enzyme make it suitable for use in food industries.

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APA: Copy

ASOODEH, AHMAD, & MOHAMMADIAN MUSAABADI, HOSSEIN. (2012). PURIFICATION AND CHARACTERIZATION OF A THERMOSTABLE NEUTROPHILIC METALLOPROTEASE FROM PSEUDOMONAS SP. DR89. IRANIAN JOURNAL OF BIOTECHNOLOGY, 10(2), 120-128. SID. https://sid.ir/paper/139226/en

Vancouver: Copy

ASOODEH AHMAD, MOHAMMADIAN MUSAABADI HOSSEIN. PURIFICATION AND CHARACTERIZATION OF A THERMOSTABLE NEUTROPHILIC METALLOPROTEASE FROM PSEUDOMONAS SP. DR89. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2012;10(2):120-128. Available from: https://sid.ir/paper/139226/en

IEEE: Copy

AHMAD ASOODEH, and HOSSEIN MOHAMMADIAN MUSAABADI, “PURIFICATION AND CHARACTERIZATION OF A THERMOSTABLE NEUTROPHILIC METALLOPROTEASE FROM PSEUDOMONAS SP. DR89,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 10, no. 2, pp. 120–128, 2012, [Online]. Available: https://sid.ir/paper/139226/en

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