SHORT COMMUNICATION: GENETIC VARIABILITY OF CALPASTATIN AND CALPAIN GENES IN IRANIAN ZEL SHEEP USING PCR-RFLP AND PCR-SSCP METHODS

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DEHNAVI ELENA; AHANI AZARI MOJTABA; HASANI SAEED; NASSIRY MOHAMMAD REZA; MOHAJER MOKHTAR; KHAN AHMADI ALI REZA

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Journal: Iranian Journal of Biotechnology Year:2012 | Volume:10 | Issue:2 Page(s): 136-139

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Title

SHORT COMMUNICATION: GENETIC VARIABILITY OF CALPASTATIN AND CALPAIN GENES IN IRANIAN ZEL SHEEP USING PCR-RFLP AND PCR-SSCP METHODS

Author(s)

Keywords

CALPASTATINQ2

CALPAINQ2

MOLECULAR METHODSQ2

POLYMORPHISMQ2

SHEEPQ3

Abstract

The genotypes for CALPASTATIN (CAST) and CALPAIN (CAPN) loci were determined by PCR-RFLP and PCRSSCP methods. Blood samples were collected from 200 pure-bred Zel SHEEP from Shirang’s Zel SHEEP Breeding Station located in south-west of Golestan, Iran. Extraction of genomic DNA was performed based on the modified salting out method. Based on results, two investigated loci were polymorphic and had different gene variants. Heterozygosis was low for both loci. Chi-square test confirmed Hardy-Weinberg equilibrium only in CAST locus using SSCP method. Detected POLYMORPHISMs and associations of genetic variation with meat production and tenderness may help to find the effective genotypes of Zel SHEEP for the economic traits.

Cites

Research Paper: Evaluating Gross Anatomy of Cervix inZel Sheep

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APA: Copy

DEHNAVI, ELENA, AHANI AZARI, MOJTABA, HASANI, SAEED, NASSIRY, MOHAMMAD REZA, MOHAJER, MOKHTAR, & KHAN AHMADI, ALI REZA. (2012). SHORT COMMUNICATION: GENETIC VARIABILITY OF CALPASTATIN AND CALPAIN GENES IN IRANIAN ZEL SHEEP USING PCR-RFLP AND PCR-SSCP METHODS. IRANIAN JOURNAL OF BIOTECHNOLOGY, 10(2), 136-139. SID. https://sid.ir/paper/139229/en

Vancouver: Copy

DEHNAVI ELENA, AHANI AZARI MOJTABA, HASANI SAEED, NASSIRY MOHAMMAD REZA, MOHAJER MOKHTAR, KHAN AHMADI ALI REZA. SHORT COMMUNICATION: GENETIC VARIABILITY OF CALPASTATIN AND CALPAIN GENES IN IRANIAN ZEL SHEEP USING PCR-RFLP AND PCR-SSCP METHODS. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2012;10(2):136-139. Available from: https://sid.ir/paper/139229/en

IEEE: Copy

ELENA DEHNAVI, MOJTABA AHANI AZARI, SAEED HASANI, MOHAMMAD REZA NASSIRY, MOKHTAR MOHAJER, and ALI REZA KHAN AHMADI, “SHORT COMMUNICATION: GENETIC VARIABILITY OF CALPASTATIN AND CALPAIN GENES IN IRANIAN ZEL SHEEP USING PCR-RFLP AND PCR-SSCP METHODS,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 10, no. 2, pp. 136–139, 2012, [Online]. Available: https://sid.ir/paper/139229/en

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