DEVELOPMENT OF A HIGH-RESOLUTION MELTING METHOD FOR SCREENING R188H POLYMORPHISM IN XRCC2 GENE

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FAYAZ SHIMA; FARD ESFAHANI PEZHMAN; KHAGHANI SHAHNAZ

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Journal: Iranian Journal of Biotechnology Year:2013 | Volume:11 | Issue:2 (42) Page(s): 104-108

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Title

DEVELOPMENT OF A HIGH-RESOLUTION MELTING METHOD FOR SCREENING R188H POLYMORPHISM IN XRCC2 GENE

Author(s)

FAYAZ SHIMA | FARD ESFAHANI PEZHMAN | KHAGHANI SHAHNAZ | Issue Writer Certificate

Keywords

HRMQ2

XRCC2Q2

GENOTYPINGQ2

Abstract

Background: The High Resolution Melting (HRM) method is a new scanning method for detecting unknown changes in DNA and its advantages have persuaded researchers to recruit it as a screening method. Objectives: Here, we developed a HRM method to screen R188H SNP (rs3218536) of XRCC2 and compared the results with a well known PCRRFLP technique.Materials and Methods: Genomic DNA samples from 350 healthy individuals were obtained. PCR-HRM analysis and PCR-RFLP method were performed simultaneously.Results: Three different melting profiles corresponding to three different genotypes recognized by HRM analysis. The results of PCR-RFLP showed no discrepancy. Conclusions: We concluded that the HRM technique can be used as a screening method for rapid discrimination of R188H genotypes in XRCC2 gene.

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APA: Copy

FAYAZ, SHIMA, FARD ESFAHANI, PEZHMAN, & KHAGHANI, SHAHNAZ. (2013). DEVELOPMENT OF A HIGH-RESOLUTION MELTING METHOD FOR SCREENING R188H POLYMORPHISM IN XRCC2 GENE. IRANIAN JOURNAL OF BIOTECHNOLOGY, 11(2 (42)), 104-108. SID. https://sid.ir/paper/139306/en

Vancouver: Copy

FAYAZ SHIMA, FARD ESFAHANI PEZHMAN, KHAGHANI SHAHNAZ. DEVELOPMENT OF A HIGH-RESOLUTION MELTING METHOD FOR SCREENING R188H POLYMORPHISM IN XRCC2 GENE. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2013;11(2 (42)):104-108. Available from: https://sid.ir/paper/139306/en

IEEE: Copy

SHIMA FAYAZ, PEZHMAN FARD ESFAHANI, and SHAHNAZ KHAGHANI, “DEVELOPMENT OF A HIGH-RESOLUTION MELTING METHOD FOR SCREENING R188H POLYMORPHISM IN XRCC2 GENE,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 11, no. 2 (42), pp. 104–108, 2013, [Online]. Available: https://sid.ir/paper/139306/en

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