INTRACELLULAR LOCALIZATION OF FLAG-PEROXISOMAL PROTEIN IN CHINESE HAMSTER OVARY (CHO) CELLS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

NAZARI JAHANTIGH M.; GHAEDI K.; NASRESFAHANI MOHAMMAD HOSSEIN; TANHAEI S.; RABIEI F.; KARBALAEI KH.; OSTAD SHARIF M.; NEMAT ELAHI MARZIEH; BAHARVAND HOSSEIN; RAZAVI SHAHNAZ; MIROULIAEI M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Biotechnology Year:2008 | Volume:6 | Issue:3 Page(s): 174-180

Download Full-Text

Persian Verion

View:

1,097

Download:

207

Cites:

Information Journal Paper

Title

INTRACELLULAR LOCALIZATION OF FLAG-PEROXISOMAL PROTEIN IN CHINESE HAMSTER OVARY (CHO) CELLS

Author(s)

Keywords

PEP CDNAQ3

PEROXISOMESQ3

PTS1 SIGNALQ3

TRANSFECTIONQ3

Abstract

Epitope tagging is a method of expressing proteins whereby an epitope for a specific monoclonal antibody is fused to a target protein using recombinant DNA techniques. The aim of this study was to sub-clone the peroxisomal protein (PEP) cDNA into a mammalian expression vector leading to the formation of a chimeric PEP-cDNA containing the FLAG epitope. The FLAG-PEP recombinant cDNA was constructed using the method of splicing by overlap extension polymerase chain reaction (SOE PCR) and inserted into the pUcD2SRaMCSHyg eukaryotic expression vector. To investigate the intracellular localization of the PEP protein that was linked to the FLAG tandem, the constructed plasmid was used for transient TRANSFECTION of the Chinese hamster Ovary (CHO) cells. The CHO cells that were transfected with the recombinant plasmid showed peroxisomal localization of FLAG-PEP as was previously shown for catalase.

Cites

No record.

References

Cite

APA: Copy

NAZARI JAHANTIGH, M., GHAEDI, K., NASRESFAHANI, MOHAMMAD HOSSEIN, TANHAEI, S., RABIEI, F., KARBALAEI, KH., OSTAD SHARIF, M., NEMAT ELAHI, MARZIEH, BAHARVAND, HOSSEIN, RAZAVI, SHAHNAZ, & MIROULIAEI, M.. (2008). INTRACELLULAR LOCALIZATION OF FLAG-PEROXISOMAL PROTEIN IN CHINESE HAMSTER OVARY (CHO) CELLS. IRANIAN JOURNAL OF BIOTECHNOLOGY, 6(3), 174-180. SID. https://sid.ir/paper/139327/en

Vancouver: Copy

NAZARI JAHANTIGH M., GHAEDI K., NASRESFAHANI MOHAMMAD HOSSEIN, TANHAEI S., RABIEI F., KARBALAEI KH., OSTAD SHARIF M., NEMAT ELAHI MARZIEH, BAHARVAND HOSSEIN, RAZAVI SHAHNAZ, MIROULIAEI M.. INTRACELLULAR LOCALIZATION OF FLAG-PEROXISOMAL PROTEIN IN CHINESE HAMSTER OVARY (CHO) CELLS. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2008;6(3):174-180. Available from: https://sid.ir/paper/139327/en

IEEE: Copy

M. NAZARI JAHANTIGH, K. GHAEDI, MOHAMMAD HOSSEIN NASRESFAHANI, S. TANHAEI, F. RABIEI, KH. KARBALAEI, M. OSTAD SHARIF, MARZIEH NEMAT ELAHI, HOSSEIN BAHARVAND, SHAHNAZ RAZAVI, and M. MIROULIAEI, “INTRACELLULAR LOCALIZATION OF FLAG-PEROXISOMAL PROTEIN IN CHINESE HAMSTER OVARY (CHO) CELLS,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 6, no. 3, pp. 174–180, 2008, [Online]. Available: https://sid.ir/paper/139327/en

Related Journal Papers