GRAPEVINE (VITIS VINIFERA L.) REGENERATION VIA SOMATIC EMBRYOGENESIS FROM WHOLE FLOWER EXPLANT

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EBADI A.; JAMAL MAHMOOD A.; MIRMASOOMI M.; OMIDI M.

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Journal: Journal of Horticultural Science Year:2012 | Volume:25 | Issue:4 Page(s): 417-424

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Title

GRAPEVINE (VITIS VINIFERA L.) REGENERATION VIA SOMATIC EMBRYOGENESIS FROM WHOLE FLOWER EXPLANT

Author(s)

EBADI A. | JAMAL MAHMOOD A. | MIRMASOOMI M. | OMIDI M. | Issue Writer Certificate

Keywords

GRAPEVINEQ2

EMBRYOGENIC CALLUSQ3

SOMATIC EMBRYOQ3

PLANTLETQ2

Abstract

To produce EMBRYOGENIC CALLUS and SOMATIC EMBRYO, whole flower explants were collected at two sampling stages of I, III and MS medium supplemented with 5 and 10mm 2, 4-D and 2mm BAP were used. For embryo differentiation, MS medium with 0.5mg/l IBA, MS medium without any plant growth regulators, MS medium with 2mg/l IBA and 0.2mg/l BAP, MS medium with 5mm 2, 4-D and 1mm BAP and finally MS medium with 2mg/l BAP were used. At embryo germination stage, MS medium with 1mg/l BAP and NN medium and cold treatment for 2 weeks were used. Results showed that in all studied cultivar, collection of whole flower explants at first sampling time resulted in highest percent of EMBRYOGENIC CALLUS and SOMATIC EMBRYO production. Response of explants to media were affected by Genotype, as Yaghouti, Bidaneh Sefid and Flame Seedless in MS medium supplemented with 5mm 2, 4-D and 1mm BAP produced highest percent of somatic EMBRYOGENIC CALLUS. However, Shahroodi did better in MS medium supplemented with 5 or 2mm BAP, and Askari respond well to MS medium supplemented with 10mm 2, 4-D and 2mm BAP. Once again, at embryo differentiation stage, each cultivar produced the highest percent of SOMATIC EMBRYO in particular medium. For embryo germination and PLANTLETs production, MS medium supplemented with 1mm BAP without chilling was best for Bidaneh Sefid, Yaghouti and Shahroodi, whereas Askari and Flame Seedless did best embryo germination and PLANTLET regeneration in MS medium supplemented with 1mm BAP accompanied by chilling treatment.

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APA: Copy

EBADI, A., JAMAL MAHMOOD, A., MIRMASOOMI, M., & OMIDI, M.. (2012). GRAPEVINE (VITIS VINIFERA L.) REGENERATION VIA SOMATIC EMBRYOGENESIS FROM WHOLE FLOWER EXPLANT. JOURNAL OF HORTICULTURE SCIENCE (AGRICULTURAL SCIENCES AND TECHNOLOGY), 25(4), 417-424. SID. https://sid.ir/paper/142495/en

Vancouver: Copy

EBADI A., JAMAL MAHMOOD A., MIRMASOOMI M., OMIDI M.. GRAPEVINE (VITIS VINIFERA L.) REGENERATION VIA SOMATIC EMBRYOGENESIS FROM WHOLE FLOWER EXPLANT. JOURNAL OF HORTICULTURE SCIENCE (AGRICULTURAL SCIENCES AND TECHNOLOGY)[Internet]. 2012;25(4):417-424. Available from: https://sid.ir/paper/142495/en

IEEE: Copy

A. EBADI, A. JAMAL MAHMOOD, M. MIRMASOOMI, and M. OMIDI, “GRAPEVINE (VITIS VINIFERA L.) REGENERATION VIA SOMATIC EMBRYOGENESIS FROM WHOLE FLOWER EXPLANT,” JOURNAL OF HORTICULTURE SCIENCE (AGRICULTURAL SCIENCES AND TECHNOLOGY), vol. 25, no. 4, pp. 417–424, 2012, [Online]. Available: https://sid.ir/paper/142495/en

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