INVESTIGATION EFFECTS OF TYPE AND CONCENTRATION OF CYTOKININ ON IN VITRO SHOOT MULTIPLICATION AND HYPERHYDRICITY OF CARNATION (DIANTHUS CARYOPHYLLUS L.)

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KHARRAZI S.M.; NEMATI S.H.; TEHRANIFAR A.; SHARIFI A.; BAGHERI A.

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Journal: Journal of Horticultural Science Year:2012 | Volume:25 | Issue:4 Page(s): 376-383

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Title

INVESTIGATION EFFECTS OF TYPE AND CONCENTRATION OF CYTOKININ ON IN VITRO SHOOT MULTIPLICATION AND HYPERHYDRICITY OF CARNATION (DIANTHUS CARYOPHYLLUS L.)

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Keywords

CARNATIONQ3

BENZYL ADENINEQ2

KINETINQ3

HYPERHYDRICITYQ2

SHOOT MULTIPLICATIONQ3

MICROPROPAGATIONQ1

Abstract

CARNATION (Dianthus caryophyllus L.) is the third most important cut flowers in the world. Tissue culture techniques offer an efficient method for MICROPROPAGATION of this ornamental plant. In present work effects of KINETIN (Kin) and BENZYL ADENINE (BA), on SHOOT MULTIPLICATION and HYPERHYDRICITY of four CARNATION cultivars (Prado Aquila K gr, Skimo Mogr, Mondeo K gr and In nove Orange Bogr) were studied. Explants from nodal segments were cultured on MS medium supplemented with different concentrations of BA (1, 2, 3 and 4 mg/l) and Kin (1, 2, 3 and 4 mg/l) in combination with 0.2 mg/l NAA, 30 g/l sucrose and 8 g/l agar. Rooting of regenerated shoots was done in the MS medium supplemented with 1 mg/l NAA. Results indicated that there were significant differences among cultivars shoot regeneration. Eskimo and Prado Aquila K gr with 3.2 and 1.5 shoots had the highest and lowest regeneration rate, respectively. Increasing the concentration of cytokinin from 1 mg/l to 4 mg/l lead to increased regenerated shoot number from 1.7 to 2.4 shoots per explant and increased HYPERHYDRICITY from 12% to 54%. In addition height of regenerated shoots were decreased by high concentration of cytokinin, especially BA. Based on HYPERHYDRICITY percentage of regenerated shoots, there was a significant difference between cultivars and cytokinins. Mondeo Kgr and Prado Aquila Kgr showed the highest (44%) and the lowest (23%) HYPERHYDRICITY, respectively and explants cultured in medium supplemented with BA caused more (40%) HYPERHYDRICITY than Kin (26%). Results of present work showed that by increasing the concentration of cytokinin specially BA, multiplication will be increased but also it will increase the HYPERHYDRICITY of plantlets and consequently it will lead to death of them. By considering the amount of multiplication and frequency of HYPERHYDRICITY for obtaining the highest number of normal shoots, using of MS medium containing 1 mg/l BA in combination with 0.2 mg/l NAA is suggested.

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APA: Copy

KHARRAZI, S.M., NEMATI, S.H., TEHRANIFAR, A., SHARIFI, A., & BAGHERI, A.. (2012). INVESTIGATION EFFECTS OF TYPE AND CONCENTRATION OF CYTOKININ ON IN VITRO SHOOT MULTIPLICATION AND HYPERHYDRICITY OF CARNATION (DIANTHUS CARYOPHYLLUS L.). JOURNAL OF HORTICULTURE SCIENCE (AGRICULTURAL SCIENCES AND TECHNOLOGY), 25(4), 376-383. SID. https://sid.ir/paper/142498/en

Vancouver: Copy

KHARRAZI S.M., NEMATI S.H., TEHRANIFAR A., SHARIFI A., BAGHERI A.. INVESTIGATION EFFECTS OF TYPE AND CONCENTRATION OF CYTOKININ ON IN VITRO SHOOT MULTIPLICATION AND HYPERHYDRICITY OF CARNATION (DIANTHUS CARYOPHYLLUS L.). JOURNAL OF HORTICULTURE SCIENCE (AGRICULTURAL SCIENCES AND TECHNOLOGY)[Internet]. 2012;25(4):376-383. Available from: https://sid.ir/paper/142498/en

IEEE: Copy

S.M. KHARRAZI, S.H. NEMATI, A. TEHRANIFAR, A. SHARIFI, and A. BAGHERI, “INVESTIGATION EFFECTS OF TYPE AND CONCENTRATION OF CYTOKININ ON IN VITRO SHOOT MULTIPLICATION AND HYPERHYDRICITY OF CARNATION (DIANTHUS CARYOPHYLLUS L.),” JOURNAL OF HORTICULTURE SCIENCE (AGRICULTURAL SCIENCES AND TECHNOLOGY), vol. 25, no. 4, pp. 376–383, 2012, [Online]. Available: https://sid.ir/paper/142498/en

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