DETECTION OF BACILLUS COAGULANS IN PROBIOTIC PRODUCTS BY PCR MOLECULAR METHOD

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MOTAZEDI F.; NIKOOPOUR H.; SHAHHOSSEINY M.H.

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Journal: Journal of Food Technology and Nutrition Year:2019 | Volume:16 | Issue:1 (61) Page(s): 113-120

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Title

DETECTION OF BACILLUS COAGULANS IN PROBIOTIC PRODUCTS BY PCR MOLECULAR METHOD

Author(s)

MOTAZEDI F. | NIKOOPOUR H. | SHAHHOSSEINY M.H. | Issue Writer Certificate

Keywords

BACILLUS COAGULANSQ1

PCRQ2

PROBIOTICSQ2

Abstract

Introduction: PROBIOTICS are microorganisms that if administered in adequate amounts would have beneficial effects on the host's health.BACILLUS COAGULANS is a probiotic bacteria which sometimes mistakenly called Lactobacillus sporogenes. B. coagulans is a gram positive and facultative aerobic and anaerobic, catalase positive, oxidase negative and endospore forming bacteria. The aim of this study is the rapid molecular detection ofB. coagulans in various probiotic products.Materials and Methods: PCR test was optimized by using B. coagulans standard strain.Limit of detection (LOD) and specificity of this test were investigated by using related species of DNA. Samples of probiotic products which contain B. coagulans were collected from the Iranian market and various methods of DNA extraction were optimized for these products.Results: Amplicon with the size of 450bp was amplified by PCR test and observed by electrophoresis method. In specificity test none were positive using DNA for intended organisms.B. coagulans species were identified in all the samples by PCR method.Conclusion: According to the results of this study B. coagulans was detected in all samples.It was concluded that PCR is a reliable and accurate method to identify these products that might contain B. coagulans PROBIOTICS bactria.

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APA: Copy

MOTAZEDI, F., NIKOOPOUR, H., & SHAHHOSSEINY, M.H.. (2019). DETECTION OF BACILLUS COAGULANS IN PROBIOTIC PRODUCTS BY PCR MOLECULAR METHOD. JOURNAL OF FOOD TECHNOLOGY AND NUTRITION, 16(1 (61) ), 113-120. SID. https://sid.ir/paper/143439/en

Vancouver: Copy

MOTAZEDI F., NIKOOPOUR H., SHAHHOSSEINY M.H.. DETECTION OF BACILLUS COAGULANS IN PROBIOTIC PRODUCTS BY PCR MOLECULAR METHOD. JOURNAL OF FOOD TECHNOLOGY AND NUTRITION[Internet]. 2019;16(1 (61) ):113-120. Available from: https://sid.ir/paper/143439/en

IEEE: Copy

F. MOTAZEDI, H. NIKOOPOUR, and M.H. SHAHHOSSEINY, “DETECTION OF BACILLUS COAGULANS IN PROBIOTIC PRODUCTS BY PCR MOLECULAR METHOD,” JOURNAL OF FOOD TECHNOLOGY AND NUTRITION, vol. 16, no. 1 (61) , pp. 113–120, 2019, [Online]. Available: https://sid.ir/paper/143439/en

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