ALLELIC VARIATION OF HARDNESS GENES (PUROINDOLINE A AND B) IN IRANIAN COMMERCIAL AND LANDRACE WHEATS

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MALEKZADEH KH.; SHAHRIARI F.A.; FARSI MOHAMMAD; MOHSENIFARD E.

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Journal: Journal of Water and Soil Science Year:2008 | Volume:12 | Issue:45 (B) Page(s): 649-654

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Title

ALLELIC VARIATION OF HARDNESS GENES (PUROINDOLINE A AND B) IN IRANIAN COMMERCIAL AND LANDRACE WHEATS

Author(s)

MALEKZADEH KH. | SHAHRIARI F.A. | FARSI MOHAMMAD | MOHSENIFARD E. | Issue Writer Certificate

Keywords

GRAIN HARDNESSQ3

PUROINDOLINEQ3

TRITICUMQ3

WHEATQ3

Abstract

Kernel hardness is one of the most important characterizations on end-use quality of bread WHEAT and also used for their marketing classification. Kernel texture, mainly controlled by one major locus (Ha) located on the short arm of chromosome 5D. Two tightly linked genes as puroindolin a, and b covered by this major locus and designed as Pina and Pinb respectively. When both PUROINDOLINEs are in their ‘functional’ wild state, grain texture is soft. When either of the PUROINDOLINE alleles is absent or alter by mutation, then the result is hard texture. In this study, 61 Iranian commercial cultivars and 92 landraces were investigated for their kernel hardness and PUROINDOLINE alleles using SKCS and, PCR and cleaved amplified polymorphic sequences (CAPS) techniques respectively. Specific primers were used to amplify Pina and Pinb. The results indicated that frequency of hard, mixed and soft genotypes were 65.6, 19.6 and 14.8% respectively, in commercial cultivars and 58.7, 13 and 28.3% in landraces varieties. Among hard type of commercial cultivars, 18 and 5, genotypes have identified as Pina-D1b and Pinb-D1b respectively. Kavir was only cultivar with Pinb-D1e allele. Pinb-D1b allele was identified in two hard types of landrace varieties. Surprisingly, Pinb-D1c was not found in any varieties. Influence of the above proindoline alleles on kernel hardness showed that the SKCS hardness index of Pina-D1b was significantly higher than that of Pinb-D1b. Our knowledge about the genetic basis of kernel hardness could provide useful information in breeding programs of bread WHEAT.

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APA: Copy

MALEKZADEH, KH., SHAHRIARI, F.A., FARSI, MOHAMMAD, & MOHSENIFARD, E.. (2008). ALLELIC VARIATION OF HARDNESS GENES (PUROINDOLINE A AND B) IN IRANIAN COMMERCIAL AND LANDRACE WHEATS. WATER AND SOIL SCIENCE (JOURNAL OF SCIENCE AND TECHNOLOGY OF AGRICULTURE AND NATURAL RESOURCES), 12(45 (B)), 649-654. SID. https://sid.ir/paper/14729/en

Vancouver: Copy

MALEKZADEH KH., SHAHRIARI F.A., FARSI MOHAMMAD, MOHSENIFARD E.. ALLELIC VARIATION OF HARDNESS GENES (PUROINDOLINE A AND B) IN IRANIAN COMMERCIAL AND LANDRACE WHEATS. WATER AND SOIL SCIENCE (JOURNAL OF SCIENCE AND TECHNOLOGY OF AGRICULTURE AND NATURAL RESOURCES)[Internet]. 2008;12(45 (B)):649-654. Available from: https://sid.ir/paper/14729/en

IEEE: Copy

KH. MALEKZADEH, F.A. SHAHRIARI, MOHAMMAD FARSI, and E. MOHSENIFARD, “ALLELIC VARIATION OF HARDNESS GENES (PUROINDOLINE A AND B) IN IRANIAN COMMERCIAL AND LANDRACE WHEATS,” WATER AND SOIL SCIENCE (JOURNAL OF SCIENCE AND TECHNOLOGY OF AGRICULTURE AND NATURAL RESOURCES), vol. 12, no. 45 (B), pp. 649–654, 2008, [Online]. Available: https://sid.ir/paper/14729/en

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