EFFECT OF EXPLANT TYPE AND DIFFERENT HORMONAL COMBINATIONS ON DIRECT REGENERATION OF LEMON BALM (MELISSA OFFICINALIS L.)

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JANGJOU KHADIJEH; HASSANI ABBAS; HOSSEINI BAHMAN; JAFARI MORAD; ALIZADEH MORTEZA

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Journal: Iranian Journal of Horticultural Science Year:2015 | Volume:45 | Issue:4 Page(s): 391-399

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Title

EFFECT OF EXPLANT TYPE AND DIFFERENT HORMONAL COMBINATIONS ON DIRECT REGENERATION OF LEMON BALM (MELISSA OFFICINALIS L.)

Author(s)

Keywords

BAPQ2

DIRECT REGENERATIONQ2

LEMON BALM (MELISSA OFFICINALIS L.)Q2

MS MEDIUMQ1

NODAL SEGMENTQ2

Abstract

Lemon balm (Melissa officinalis L.) is an herbaceous perennial plant of the Lamiaceae family that has a wide edible and medicinal uses. In this research the potential of five different explants (nodal segments, shoot tip, cotyledon, hypocotyl and leaf segments) on MS MEDIUM supplemented with different concentrations of 6-Benzylaminopurine (BAP) (2.2, 4.4 and 8.8 mm) alone or in combination with 1mm Indoleacetic acid (IAA) for DIRECT REGENERATION of Lemon balm was investigated. The experiment was conducted using a factorial based on completely randomized design with three replications. The maximum mean number of shoots (23.59 and 16.90 shoots in shoot tip and NODAL SEGMENT explants, respectively) was observed on the MS MEDIUM supplemented with 8.8 mm BAP. The highest regeneration rate (99%) in shoot tip and NODAL SEGMENT explants was achieved on the MS MEDIUM supplemented with 8.8 and 2.2 mm BAP, respectively. No shoot regeneration occurred in other explants. The rooting of regenerated shoots was assessed on MS, 1.2 MS and MS MEDIUM supplemented with 1, 2.5, 4.92 and 9.84mm Indole butyric acid (IBA). The highest mean number of roots (9.06 roots per explants) was observed on the MS MEDIUM supplemented with 4.92 mm IBA.

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APA: Copy

JANGJOU, KHADIJEH, HASSANI, ABBAS, HOSSEINI, BAHMAN, JAFARI, MORAD, & ALIZADEH, MORTEZA. (2015). EFFECT OF EXPLANT TYPE AND DIFFERENT HORMONAL COMBINATIONS ON DIRECT REGENERATION OF LEMON BALM (MELISSA OFFICINALIS L.). IRANIAN JOURNAL OF HORTICULTURAL SCIENCES (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), 45(4), 391-399. SID. https://sid.ir/paper/148088/en

Vancouver: Copy

JANGJOU KHADIJEH, HASSANI ABBAS, HOSSEINI BAHMAN, JAFARI MORAD, ALIZADEH MORTEZA. EFFECT OF EXPLANT TYPE AND DIFFERENT HORMONAL COMBINATIONS ON DIRECT REGENERATION OF LEMON BALM (MELISSA OFFICINALIS L.). IRANIAN JOURNAL OF HORTICULTURAL SCIENCES (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES)[Internet]. 2015;45(4):391-399. Available from: https://sid.ir/paper/148088/en

IEEE: Copy

KHADIJEH JANGJOU, ABBAS HASSANI, BAHMAN HOSSEINI, MORAD JAFARI, and MORTEZA ALIZADEH, “EFFECT OF EXPLANT TYPE AND DIFFERENT HORMONAL COMBINATIONS ON DIRECT REGENERATION OF LEMON BALM (MELISSA OFFICINALIS L.),” IRANIAN JOURNAL OF HORTICULTURAL SCIENCES (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), vol. 45, no. 4, pp. 391–399, 2015, [Online]. Available: https://sid.ir/paper/148088/en

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