REGENERATION OF MINIATURE ROSE (ROSA HYBRIDA,. CV 'TANBAKEDA') THROUGH SOMATIC EMBRYOGENESIS

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VISHLAGHI N.; JALALI JAVARAN M.; MOEINI A.

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Journal: Iranian Journal of Horticultural Science Year:2010 | Volume:41 | Issue:4 Page(s): 347-357

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Information Journal Paper

Title

REGENERATION OF MINIATURE ROSE (ROSA HYBRIDA,. CV 'TANBAKEDA') THROUGH SOMATIC EMBRYOGENESIS

Author(s)

VISHLAGHI N. | JALALI JAVARAN M. | MOEINI A. | Issue Writer Certificate

Keywords

EMBRYOGENESISQ3

CALLUS INDUCTIONQ3

REGENERATIONQ3

ROSA HYBRIDQ3

'CV TANBAKEDA’Q3

Abstract

Rose is considered as one of the most popular and important ornamental plants in the world. During the last decade, tissue culture, micropropagation as well as genetic engineering have prevailed in rose production industry. Gene transfer to embryogenic callus, derived from different types of explants is currently one of the most popular methods of improvement of rose cultivars, presented in particular as cut flower with such novel characteristics as blue color. An efficient tissue culture procedure covering high frequency embryogenic CALLUS INDUCTION and plant REGENERATION is pre-requisite for every successful genetic engineering program. An efficient method for embryogenic CALLUS INDUCTION from growing nodal stem segments on MS medium followed by a selection of appropriate young leaflets is hereby reported. MS medium complemented with 2 mg/L 2, 4-D, and 0.5 mg/L NAA as wekk as medium B5 complemented with 0.5 mg/L 2, 4-D and 2.5 mg/L NAA plus 0.5mg/L kinetin were used for CALLUS INDUCTION. Cali were then subcultured in MS medium with higher levels of hormone (3 mg/L 2, 4-D and 0.5 mg/LNAA) to develop suitable size and shape embryos. This was followed by transferring the calli onto an MS medium complemented with 8 hormonal treatments. MS medium along with 1 mg/L of TDZ resulted in a highest number of embryos. High frequency of shoot development from mature somatic embryo and subsequent plant REGENERATION were obtained on a medium containing 3 mg/L of BAP. Shoots were rooted on MS medium supplemented with three various hormonal treatments, and there were no significant differences observed between treatments.

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APA: Copy

VISHLAGHI, N., JALALI JAVARAN, M., & MOEINI, A.. (2010). REGENERATION OF MINIATURE ROSE (ROSA HYBRIDA,. CV 'TANBAKEDA') THROUGH SOMATIC EMBRYOGENESIS. IRANIAN JOURNAL OF HORTICULTURAL SCIENCES (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), 41(4), 347-357. SID. https://sid.ir/paper/148262/en

Vancouver: Copy

VISHLAGHI N., JALALI JAVARAN M., MOEINI A.. REGENERATION OF MINIATURE ROSE (ROSA HYBRIDA,. CV 'TANBAKEDA') THROUGH SOMATIC EMBRYOGENESIS. IRANIAN JOURNAL OF HORTICULTURAL SCIENCES (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES)[Internet]. 2010;41(4):347-357. Available from: https://sid.ir/paper/148262/en

IEEE: Copy

N. VISHLAGHI, M. JALALI JAVARAN, and A. MOEINI, “REGENERATION OF MINIATURE ROSE (ROSA HYBRIDA,. CV 'TANBAKEDA') THROUGH SOMATIC EMBRYOGENESIS,” IRANIAN JOURNAL OF HORTICULTURAL SCIENCES (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), vol. 41, no. 4, pp. 347–357, 2010, [Online]. Available: https://sid.ir/paper/148262/en

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