MOLECULAR DETECTION OF CLOSTRIDIUM PERFRINGENS IN SOME RAW ANIMAL FOOD ORIGIN PRODUCTS IN SHAHREKORD, 2014

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SHAKERIAN AMIR; RAHIMI EBRAHIM; MESBAH JAMAL; MOUSAVI MOHAMMAD

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Journal: Payavard Salamat Year:2017 | Volume:11 | Issue:4 Page(s): 391-399

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Title

MOLECULAR DETECTION OF CLOSTRIDIUM PERFRINGENS IN SOME RAW ANIMAL FOOD ORIGIN PRODUCTS IN SHAHREKORD, 2014

Author(s)

SHAKERIAN AMIR | RAHIMI EBRAHIM | MESBAH JAMAL | MOUSAVI MOHAMMAD | Issue Writer Certificate

Keywords

CLOSTRIDIUM PERFRINGENSQ1

CURDQ1

MEATQ1

PCRQ1

Abstract

Background and Aim: CLOSTRIDIUM PERFRINGENS is one of the major agents of food poisoning in humans around the world. This study was accomplished to identify and track the toxins of CLOSTRIDIUM PERFRINGENS bacterium in some raw animal food origin products in Shahrekord.Materials and Methods: In this study, 100 samples of traditional and commercial CURD and 43 samples of beef and lamb MEATs were randomly collected from Shahrekord’s shopping center in 2014. Then to identify the bacteria in samples cultivation and polymerase chain reaction (PCR) method were used.Results: In culture method no positive samples were isolated. However, frequency of CLOSTRIDIUM PERFRINGENS bacterium in traditional and commercial CURD samples and beef and lambs samples have been reported as 6, 10, 25 and 13 percent using PCR method, respectively. Frequency ofcpa, cpb, cpe, cpi and etx genes were detected in CURD samples were 37.5, 25, 75, 12.5 and 12.5 percent respectively and in MEAT samples 62, 50, 75.5, 37.5 and 25 percent respectively. Statistically significant differences were observed between the prevalence of the bacterium in various samples and their isolated toxins (p<0.05).Conclusion: With attention to infection of above food samples to CLOSTRIDIUM PERFRINGENS and also its transfer by foodstuffs to human, it is necessary to reduce food pollution of this organism for of public health.

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APA: Copy

SHAKERIAN, AMIR, RAHIMI, EBRAHIM, MESBAH, JAMAL, & MOUSAVI, MOHAMMAD. (2017). MOLECULAR DETECTION OF CLOSTRIDIUM PERFRINGENS IN SOME RAW ANIMAL FOOD ORIGIN PRODUCTS IN SHAHREKORD, 2014. PAYAVARD-SALAMAT, 11(4 ), 391-399. SID. https://sid.ir/paper/149760/en

Vancouver: Copy

SHAKERIAN AMIR, RAHIMI EBRAHIM, MESBAH JAMAL, MOUSAVI MOHAMMAD. MOLECULAR DETECTION OF CLOSTRIDIUM PERFRINGENS IN SOME RAW ANIMAL FOOD ORIGIN PRODUCTS IN SHAHREKORD, 2014. PAYAVARD-SALAMAT[Internet]. 2017;11(4 ):391-399. Available from: https://sid.ir/paper/149760/en

IEEE: Copy

AMIR SHAKERIAN, EBRAHIM RAHIMI, JAMAL MESBAH, and MOHAMMAD MOUSAVI, “MOLECULAR DETECTION OF CLOSTRIDIUM PERFRINGENS IN SOME RAW ANIMAL FOOD ORIGIN PRODUCTS IN SHAHREKORD, 2014,” PAYAVARD-SALAMAT, vol. 11, no. 4 , pp. 391–399, 2017, [Online]. Available: https://sid.ir/paper/149760/en

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