Experimental study of partitioning of-Amylase in aqueous two phase systems based on Poly (ethylene) glycol 2000/ Magnesium sulfate

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Dehnavi Seyed Mohsen; HADIDI NAGHMEH; Pazuki Gholamreza; Vossoughi Manouchehr

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Journal: JOURNAL OF SEPARATION SCIENCE AND ENGINEERING Year:2018 | Volume:10 | Issue:2 Page(s): 80-87

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Title

Experimental study of partitioning of-Amylase in aqueous two phase systems based on Poly (ethylene) glycol 2000/ Magnesium sulfate

Author(s)

Dehnavi Seyed Mohsen | HADIDI NAGHMEH | Pazuki Gholamreza | Vossoughi Manouchehr | Issue Writer Certificate

Keywords

a-amylaseQ1

Aqueous two phase systemsQ1

Partition coefficientQ1

Poly (ethylene glycol) 2000Q1

Magnesium sulfate saltQ1

Abstract

In recent years, the Aqueous two phase systems (ATPS) are applied for separation of biological products as a new method. In this research, the ATPS based on poly (ethylene glycol) 2000 and magnesium sulfate are applied for separation of-amylase. The binodal curve is firstly obtained by using cloud point method at 23 and is correlated by the Merchuk equation. Also, partitioning of-amylase in two phase region at various working points is investigated and tie lines, Partition coefficients and recovery are obtained. The results show that the bimodal curve is fitted with the Merchuk equation well. After partitioning of-amylase enzyme in the ATPS,-amylase tends to the top phase and the average of the Partition coefficients the extraction recoveries are 1. 15 and 71%, respectively. Also, the Partition coefficient of-amylase is increased with increasing concentration of polymer (at constant concentration of salt) and concentration of salt (at constant concentration of polymer). The values of Gibbs free energy of-amylase show that partitioning process is spontaneously toward top phase.

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APA: Copy

Dehnavi, Seyed Mohsen, HADIDI, NAGHMEH, Pazuki, Gholamreza, & Vossoughi, Manouchehr. (2018). Experimental study of partitioning of-Amylase in aqueous two phase systems based on Poly (ethylene) glycol 2000/ Magnesium sulfate. JOURNAL OF SEPARATION SCIENCE AND ENGINEERING, 10(2 ), 80-87. SID. https://sid.ir/paper/150060/en

Vancouver: Copy

Dehnavi Seyed Mohsen, HADIDI NAGHMEH, Pazuki Gholamreza, Vossoughi Manouchehr. Experimental study of partitioning of-Amylase in aqueous two phase systems based on Poly (ethylene) glycol 2000/ Magnesium sulfate. JOURNAL OF SEPARATION SCIENCE AND ENGINEERING[Internet]. 2018;10(2 ):80-87. Available from: https://sid.ir/paper/150060/en

IEEE: Copy

Seyed Mohsen Dehnavi, NAGHMEH HADIDI, Gholamreza Pazuki, and Manouchehr Vossoughi, “Experimental study of partitioning of-Amylase in aqueous two phase systems based on Poly (ethylene) glycol 2000/ Magnesium sulfate,” JOURNAL OF SEPARATION SCIENCE AND ENGINEERING, vol. 10, no. 2 , pp. 80–87, 2018, [Online]. Available: https://sid.ir/paper/150060/en

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