PARTITIONING OF PROTEASE IN PEG/SODIUM CITRATE AND POTASSIUM CITRATE AQUEOUS TWO-PHASE SYSTEM

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KHAYATI GHOLAM; FIROOZI MOTAHAREH; AHMADI MITRA

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Journal: JOURNAL OF SEPARATION SCIENCE AND ENGINEERING Year:2016 | Volume:8 | Issue:1 Page(s): 1-6

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Title

PARTITIONING OF PROTEASE IN PEG/SODIUM CITRATE AND POTASSIUM CITRATE AQUEOUS TWO-PHASE SYSTEM

Author(s)

KHAYATI GHOLAM | FIROOZI MOTAHAREH | AHMADI MITRA | Issue Writer Certificate

Keywords

PARTITIONINGQ2

PROTEASEQ2

AQUEOUS TWO-PHASE SYSTEMQ2

POLY ETHYLENE GLYCOLQ1

SODIUM CITRATEQ2

POTASSIUM CITRATEQ2

Abstract

Purification of the desired protein from the fermentation broth containing a wide variety of biomolecules is one of the major challenges in the biotechnology industry. The aim of this work was PARTITIONING of PROTEASE from the fermentation broth using AQUEOUS TWO-PHASE SYSTEMs (ATPS). ATPS has proved to be a valuable tool for separating and purifying mixtures of biomolecules. In this study, the various AQUEOUS TWO-PHASE SYSTEMs containing polyethylene glycol (PEG 2000 and 8000) at a concentration of 40, 50, and 60% (w/w) with salts SODIUM CITRATE, POTASSIUM CITRATE at a concentration of 40 and 50% (w/w) were investigated for extraction of PROTEASE from fermentation broth. The results showed that the polyethylene glycol 2000/ SODIUM CITRATE system was suitable for the extraction of PROTEASE from fermentation broth ofBacillus licheniformisand the best operating system was contained the 40% (w/w) polyethylene glycol 2000, 40% (w/w) SODIUM CITRATE resulted in enzyme activity recovery of 48.9% and purification factor of 12.7.

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APA: Copy

KHAYATI, GHOLAM, FIROOZI, MOTAHAREH, & AHMADI, MITRA. (2016). PARTITIONING OF PROTEASE IN PEG/SODIUM CITRATE AND POTASSIUM CITRATE AQUEOUS TWO-PHASE SYSTEM. JOURNAL OF SEPARATION SCIENCE AND ENGINEERING, 8(1), 1-6. SID. https://sid.ir/paper/150065/en

Vancouver: Copy

KHAYATI GHOLAM, FIROOZI MOTAHAREH, AHMADI MITRA. PARTITIONING OF PROTEASE IN PEG/SODIUM CITRATE AND POTASSIUM CITRATE AQUEOUS TWO-PHASE SYSTEM. JOURNAL OF SEPARATION SCIENCE AND ENGINEERING[Internet]. 2016;8(1):1-6. Available from: https://sid.ir/paper/150065/en

IEEE: Copy

GHOLAM KHAYATI, MOTAHAREH FIROOZI, and MITRA AHMADI, “PARTITIONING OF PROTEASE IN PEG/SODIUM CITRATE AND POTASSIUM CITRATE AQUEOUS TWO-PHASE SYSTEM,” JOURNAL OF SEPARATION SCIENCE AND ENGINEERING, vol. 8, no. 1, pp. 1–6, 2016, [Online]. Available: https://sid.ir/paper/150065/en

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