DETERMINING OF RELATIVE POPULATION OF CLOSTRIDIUM SPP. BACTERIA IN DUODENUM, JEJUNUM, ILEUM AND CECUM OF BROILERS AT VARIOUS AGES USING DENSITOMETRY TECHNIQUE BASED ON 16S RDNA APPROACH

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SEIDAVI A.R.; CHAMANI M.

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Journal: JOURNAL OF VETERINARY MICROBIOLOGY Year:2010 | Volume:6 | Issue:1 (19) Page(s): 1-10

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Title

DETERMINING OF RELATIVE POPULATION OF CLOSTRIDIUM SPP. BACTERIA IN DUODENUM, JEJUNUM, ILEUM AND CECUM OF BROILERS AT VARIOUS AGES USING DENSITOMETRY TECHNIQUE BASED ON 16S RDNA APPROACH

Author(s)

SEIDAVI A.R. | CHAMANI M. | Issue Writer Certificate

Keywords

CLOSTRIDIUM SPP.Q2

DENSITOMETRYQ3

CECUMQ2

AVIANQ2

POLYMERASE CHAIN REACTIONQ2

Abstract

CLOSTRIDIUM SPP. especially Clostridium perfringens are pathogenic factors in human and animals. It was used novel approach based on POLYMERASE CHAIN REACTION named DENSITOMETRY technique for determining of CLOSTRIDIUM SPP. bacteria frequency in duodenum, jejunum, ileum and CECUM of broiler. Digest contents were removed and extracted their DNA. It was obtained specific bands for detecting of CLOSTRIDIUM SPP. and all bacteria using POLYMERASE CHAIN REACTIONs. Then, it was determined relative population of CLOSTRIDIUM SPP. relative to total gut bacteria by means of DENSITOMETRY technique based on 16S ribosomal DNA approach. Result analysis was showed CLOSTRIDIUM SPP. consists 0.36% of total duodenum bacteria. Also it was showed CLOSTRIDIUM SPP. consists 0.20% of total jejunum bacteria. Mean while CLOSTRIDIUM SPP. consists 1.15% of total ileum bacteria. Furthermore it was showed CLOSTRIDIUM SPP. consists 3.72% of total CECUM bacteria. Relative population of CLOSTRIDIUM SPP. in lower segments i.e. ileum and CECUM were higher than upper segments i.e. duodenum and jejunum. Meanwhile among different ages, the highest and lowest relative population of CLOSTRIDIUM SPP. obtained in 4 and 14 of ages respectively. The lowest relative population of CLOSTRIDIUM SPP. obtained in duodenum of broilers in 4d of ages (0.00016%). Also, the highest relative population of CLOSTRIDIUM SPP. obtained in CECUM of broilers in 4d of ages (4.87%). At all three studied 4, 14 and 30d of ages, the highest relative population of CLOSTRIDIUM SPP. obtained in CECUM and the lowest relative population of CLOSTRIDIUM SPP. obtained in jejunum. From obtained results, it was showed CLOSTRIDIUM SPP. populations are variable in various intestine segments and DENSITOMETRY has efficiency for determining relative population of these of bacteria.

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APA: Copy

SEIDAVI, A.R., & CHAMANI, M.. (2010). DETERMINING OF RELATIVE POPULATION OF CLOSTRIDIUM SPP. BACTERIA IN DUODENUM, JEJUNUM, ILEUM AND CECUM OF BROILERS AT VARIOUS AGES USING DENSITOMETRY TECHNIQUE BASED ON 16S RDNA APPROACH. JOURNAL OF VETERINARY MICROBIOLOGY, 6(1 (19)), 1-10. SID. https://sid.ir/paper/152019/en

Vancouver: Copy

SEIDAVI A.R., CHAMANI M.. DETERMINING OF RELATIVE POPULATION OF CLOSTRIDIUM SPP. BACTERIA IN DUODENUM, JEJUNUM, ILEUM AND CECUM OF BROILERS AT VARIOUS AGES USING DENSITOMETRY TECHNIQUE BASED ON 16S RDNA APPROACH. JOURNAL OF VETERINARY MICROBIOLOGY[Internet]. 2010;6(1 (19)):1-10. Available from: https://sid.ir/paper/152019/en

IEEE: Copy

A.R. SEIDAVI, and M. CHAMANI, “DETERMINING OF RELATIVE POPULATION OF CLOSTRIDIUM SPP. BACTERIA IN DUODENUM, JEJUNUM, ILEUM AND CECUM OF BROILERS AT VARIOUS AGES USING DENSITOMETRY TECHNIQUE BASED ON 16S RDNA APPROACH,” JOURNAL OF VETERINARY MICROBIOLOGY, vol. 6, no. 1 (19), pp. 1–10, 2010, [Online]. Available: https://sid.ir/paper/152019/en

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