IDENTIFICATION AND ISOLATION OF RUMEN FUNGI OF SISTANI CATTLE IN SISTAN

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RAKHSHANI E.; DEHGHANI M.R.; CHAMANI M.

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Journal: JOURNAL OF VETERINARY MICROBIOLOGY Year:2017 | Volume:13 | Issue:1 (34) Page(s): 121-130

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Title

IDENTIFICATION AND ISOLATION OF RUMEN FUNGI OF SISTANI CATTLE IN SISTAN

Author(s)

RAKHSHANI E. | DEHGHANI M.R. | CHAMANI M. | Issue Writer Certificate

Keywords

MORPHOLOGYQ2

RUMENQ1

ANAEROBIC FUNGIQ1

SISTANI CATTLEQ1

Abstract

The SISTANI CATTLE are indigenous breed of Sistan region. The purpose of this research was the separation and studying the appearance MORPHOLOGY of ANAEROBIC FUNGI in the SISTANI CATTLE RUMEN in Sistan region. Sampling from the solid and liquid contents of 50 SISTANI CATTLE was done randomly in Zabol slaughterhouse and these samples were used as the source of fungus to inoculation to culture. The semi-defined medium environment was used in this research to cultivation, separation and purification of ANAEROBIC FUNGI. The roll bottle method was used for purification of RUMEN fungi and the antibiotic solution (ampicillin, penicillin, streptomycin, oxytetracycline and chloramphenicol) were used for inhibiting growth of bacteria. Samples of pure fungi were transferred to culture and were observed after growth in glass slide with light microscope. With regard of morphologic characteristics the genus of Neocallimastix and species of Orpinomyces joyonni, Piromyces mae, Piromyces communis, Piromyces minutus, Piromyces rhizinflata was isolated in RUMEN of SISTANI CATTLE. With identification of these fungi species in RUMEN of SISTANI CATTLE, it is recommended to perform molecular test and enzyme extraction for more survey characteristics in future research.

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APA: Copy

RAKHSHANI, E., DEHGHANI, M.R., & CHAMANI, M.. (2017). IDENTIFICATION AND ISOLATION OF RUMEN FUNGI OF SISTANI CATTLE IN SISTAN. JOURNAL OF VETERINARY MICROBIOLOGY, 13(1 (34) ), 121-130. SID. https://sid.ir/paper/152077/en

Vancouver: Copy

RAKHSHANI E., DEHGHANI M.R., CHAMANI M.. IDENTIFICATION AND ISOLATION OF RUMEN FUNGI OF SISTANI CATTLE IN SISTAN. JOURNAL OF VETERINARY MICROBIOLOGY[Internet]. 2017;13(1 (34) ):121-130. Available from: https://sid.ir/paper/152077/en

IEEE: Copy

E. RAKHSHANI, M.R. DEHGHANI, and M. CHAMANI, “IDENTIFICATION AND ISOLATION OF RUMEN FUNGI OF SISTANI CATTLE IN SISTAN,” JOURNAL OF VETERINARY MICROBIOLOGY, vol. 13, no. 1 (34) , pp. 121–130, 2017, [Online]. Available: https://sid.ir/paper/152077/en

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