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Information Journal Paper

Title

THE EFFECT OF DIFFERENT CONCENTRATION OF GLUCOSE ON BOVINE SPERM MOTILITY PATTERN

Pages

  93-97

Abstract

 To study the effect of GLUCOSE on BOVINE EPIDIDYMAL SPERM MOTILITY patterns by computer assisted sperm analysis (CASA), testicles were isolated from slaughtered bulls in a local slaughterhouse in Tehran suburb and carried to laboratory in cool container (4oC). In laboratory, testicles were removed from tunica albugina, epididymides were incised and 4-6×106 sperm cells/ml were transferred into BO medium containing different concentrations of GLUCOSE (G1-G4) and incubated in a 5% CO2 incubator in humid air at 38 oC for up to 8 hours. Every hour sperm cells were observed under the CASA system and data for: the proportion of sperm cells with high speed, the proportion of live sperm cells and the mean straight line velocity (VSL) were recorded. The results showed that the proportions of sperm cells with high speed were significantly higher in G3 medium comparing to other groups during 2, 4 and 6 h post incubation (p<0.05). The proportion of live sperm cells were different between G3 and G1 as well as G3 and G4 1 h post incubation (p<0.05). After incubation no significant difference was observed between different groups. VSL was not either different between groups during experiment. The results of the present study showed that higher and lower doses of GLUCOSE could not support BOVINE EPIDIDYMAL SPERM MOTILITY.

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    Cite

    APA: Copy

    ASADI, E., BABAPOOR, V., & TAJIK, P.. (2011). THE EFFECT OF DIFFERENT CONCENTRATION OF GLUCOSE ON BOVINE SPERM MOTILITY PATTERN. JOURNAL OF VETERINARY MICROBIOLOGY, 7(21 (SUPPLEMENTARY)), 93-97. SID. https://sid.ir/paper/152131/en

    Vancouver: Copy

    ASADI E., BABAPOOR V., TAJIK P.. THE EFFECT OF DIFFERENT CONCENTRATION OF GLUCOSE ON BOVINE SPERM MOTILITY PATTERN. JOURNAL OF VETERINARY MICROBIOLOGY[Internet]. 2011;7(21 (SUPPLEMENTARY)):93-97. Available from: https://sid.ir/paper/152131/en

    IEEE: Copy

    E. ASADI, V. BABAPOOR, and P. TAJIK, “THE EFFECT OF DIFFERENT CONCENTRATION OF GLUCOSE ON BOVINE SPERM MOTILITY PATTERN,” JOURNAL OF VETERINARY MICROBIOLOGY, vol. 7, no. 21 (SUPPLEMENTARY), pp. 93–97, 2011, [Online]. Available: https://sid.ir/paper/152131/en

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