PRESENTING AN APPROPRIATE METHOD FOR ISOLATION OF BACTERIA FREE CRYPTOSPORIDIUM PARVUM OOCYSTS

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SHAYAN P.; ASGHARI Z.; EBRAHIMZADEH E.; OMIDIAN Z.; ZARGHAMI F.

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Journal: JOURNAL OF VETERINARY MICROBIOLOGY Year:2013 | Volume:8 | Issue:2 (25) Page(s): 75-90

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Title

PRESENTING AN APPROPRIATE METHOD FOR ISOLATION OF BACTERIA FREE CRYPTOSPORIDIUM PARVUM OOCYSTS

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Keywords

CRYPTOSPORIDIUM PARVUMQ2

OOCYSTQ2

FLOATATIONQ2

FILTRATIONQ2

Abstract

CRYPTOSPORIDIUM PARVUM is a zoonotic protozoan which causes disease in immunocompromised patients and newborn animals especially calves. The OOCYSTs excreted by infected animals are introduced into the environment and water. Since the low number of OOCYST can cause infection and there is no effective disinfectant against cryptosporidium, the investigation dealing with the cryptosporidiosis is of growing interest among many researchers. Therefore, they try to improve the existing isolation methods of C. parvum OOCYSTs even in environmental samples with small number of OOCYSTs, to obtain much purified OOCYSTs for immunological and molecular studies. Different methods have gained some success on the isolation of cryptosporidium, but they didn’t result in obtaining completely pure OOCYSTs. The aim of the present study was to try to develop an isolating system for bacteria free OOCYSTs. For this aim, four flotation methods have been used to isolate the C. parvum OOCYSTs from the feces, including NaCl FLOATATION, sucrose FLOATATION, ficoll gradient and 55% sucrose FLOATATION. A FLOATATION method based on 55% sucrose was evaluated as the best method for the primary isolation of Cryptosporidium oocytes. The nitrocellulose filter (with 3µm pore size) was used to remove bacteria and other contaminants. Our results suggest that the OOCYSTs isolated using FILTRATION were free of bacteria and the amount of pure OOCYSTs was 30%. Although the amount of isolated OOCYSTs was limited, the isolated pure OOCYSTs can be used for proteomics studies.

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APA: Copy

SHAYAN, P., ASGHARI, Z., EBRAHIMZADEH, E., OMIDIAN, Z., & ZARGHAMI, F.. (2013). PRESENTING AN APPROPRIATE METHOD FOR ISOLATION OF BACTERIA FREE CRYPTOSPORIDIUM PARVUM OOCYSTS. JOURNAL OF VETERINARY MICROBIOLOGY, 8(2 (25)), 75-90. SID. https://sid.ir/paper/152138/en

Vancouver: Copy

SHAYAN P., ASGHARI Z., EBRAHIMZADEH E., OMIDIAN Z., ZARGHAMI F.. PRESENTING AN APPROPRIATE METHOD FOR ISOLATION OF BACTERIA FREE CRYPTOSPORIDIUM PARVUM OOCYSTS. JOURNAL OF VETERINARY MICROBIOLOGY[Internet]. 2013;8(2 (25)):75-90. Available from: https://sid.ir/paper/152138/en

IEEE: Copy

P. SHAYAN, Z. ASGHARI, E. EBRAHIMZADEH, Z. OMIDIAN, and F. ZARGHAMI, “PRESENTING AN APPROPRIATE METHOD FOR ISOLATION OF BACTERIA FREE CRYPTOSPORIDIUM PARVUM OOCYSTS,” JOURNAL OF VETERINARY MICROBIOLOGY, vol. 8, no. 2 (25), pp. 75–90, 2013, [Online]. Available: https://sid.ir/paper/152138/en

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