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Information Journal Paper

Title

ISOLATION AND IDENTIFICATION OFMYCOPLASMA BOVIGENITALIUM FROM BOVINE MILK SUFFERING OF CLINICAL MASTITIS BY CULTURE AND PCR METHODS

Pages

  51-58

Abstract

 Background and Objectives: MYCOPLASMA BOVIGENITALIUM is one of the MASTITIScausing pathogen in dairy cows. The complications of the disease are reduced feeding, changes in the quality and consistency of its stiffness, swelling Cartier, arthritis, infection of the mucous membranes of the respiratory tract, urinary tract, ear infections and septicemia. The purpose of this study was to Isolation and Identification of MYCOPLASMA BOVIGENITALIUM from bovine milk suffering of clinical MASTITIS by culture and PCR methods. Materials and methods: In this descriptive study, 156 samples of milk collected from cows with clinical signs of MASTITIS in dairies industry. Samples were cultured in PPLO broth and PPLO agar. The bacteria DNAs were extracted by phenol/ chloroform method. The PCR assey applied for detection of M. bovigenitalium by molecular method used MbgF, MbgR specific primers. Results: In the culturing method from 156 samples, 57 (37%) samples were reported to be positive for Mycoplasma and 5 (3%) were suspected to be positive. In the PCR test from 156 samples, 62 (40%) samples were positive for Mycoplasma. In the specific PCR test, 1 (2%) of these specimens were positive for MYCOPLASMA BOVIGENITALIUM.Conclusion: The use of the molecular method of PCR showed that it is a rapid diagnostic method with high sensitivity and specificity, which is used as a classical method.

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    APA: Copy

    MASOUMALI NEJAD, Z., & POURBAKHSH, S.A.. (2018). ISOLATION AND IDENTIFICATION OFMYCOPLASMA BOVIGENITALIUM FROM BOVINE MILK SUFFERING OF CLINICAL MASTITIS BY CULTURE AND PCR METHODS. JOURNAL OF VETERINARY MICROBIOLOGY, 14(1 (36) ), 51-58. SID. https://sid.ir/paper/152175/en

    Vancouver: Copy

    MASOUMALI NEJAD Z., POURBAKHSH S.A.. ISOLATION AND IDENTIFICATION OFMYCOPLASMA BOVIGENITALIUM FROM BOVINE MILK SUFFERING OF CLINICAL MASTITIS BY CULTURE AND PCR METHODS. JOURNAL OF VETERINARY MICROBIOLOGY[Internet]. 2018;14(1 (36) ):51-58. Available from: https://sid.ir/paper/152175/en

    IEEE: Copy

    Z. MASOUMALI NEJAD, and S.A. POURBAKHSH, “ISOLATION AND IDENTIFICATION OFMYCOPLASMA BOVIGENITALIUM FROM BOVINE MILK SUFFERING OF CLINICAL MASTITIS BY CULTURE AND PCR METHODS,” JOURNAL OF VETERINARY MICROBIOLOGY, vol. 14, no. 1 (36) , pp. 51–58, 2018, [Online]. Available: https://sid.ir/paper/152175/en

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