PURIFICATION OF IMMUNOMODULATOR FRACTION COMPONENTS OF GARLIC (R10) BY HPLC

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GHAZANFARI T.; YARAEE ROYA; ASOUDEH A.; RAJABIAN T.; KARDAR M.; MOHAMMAD HASAN ZOHEYR; NADERIMANESH H.; SOUFIAN S.; JAMALI D.

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Journal: Journal of Medicinal Plants Year:2009 | Volume:8 | Issue:29 Page(s): 100-106

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Title

PURIFICATION OF IMMUNOMODULATOR FRACTION COMPONENTS OF GARLIC (R10) BY HPLC

Author(s)

Keywords

GARLICQ3

IMMUNOMODULATOR FRACTIONQ3

HPLCQ3

PURIFICATIONQ3

Abstract

Background: It has been reported that GARLIC extract and its components show medicinal effects including immunomodulatory activities. We have isolated the immunomodulatory fraction (R10) previously. Objective: In this study we have proposed to purify the components of R10 using HPLC.Methods: Crude GARLIC extract purchased from Hamadan, Iran. R10 fraction including 10-50 KD molecules have been isolated using ultrafiltration. Further PURIFICATION has been made using Vaydac 208Tpv10, a semi-preparative HPLC reversphase choromatography column. Tricine SDS-PAGE has been used to determine molecular weight of the samples.Results: 6 major peakes were obtained from HPLC of R10 at gradient of 0.25% of buffer B/min through 60 minute. The molecular weight of 3 peaks (samples 0, 1 and 3) was 12 KD with tricine-SDSPAGE.Conclusion: Using C8 HPLC reversphase choromatography seems to be appropriate tool for PURIFICATION of R10 components and the purified components at peak 0, 1 and 3 seems to be isotypic molecules.

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APA: Copy

GHAZANFARI, T., YARAEE, ROYA, ASOUDEH, A., RAJABIAN, T., KARDAR, M., MOHAMMAD HASAN, ZOHEYR, NADERIMANESH, H., SOUFIAN, S., & JAMALI, D.. (2009). PURIFICATION OF IMMUNOMODULATOR FRACTION COMPONENTS OF GARLIC (R10) BY HPLC. JOURNAL OF MEDICINAL PLANTS, 8(29), 100-106. SID. https://sid.ir/paper/15228/en

Vancouver: Copy

GHAZANFARI T., YARAEE ROYA, ASOUDEH A., RAJABIAN T., KARDAR M., MOHAMMAD HASAN ZOHEYR, NADERIMANESH H., SOUFIAN S., JAMALI D.. PURIFICATION OF IMMUNOMODULATOR FRACTION COMPONENTS OF GARLIC (R10) BY HPLC. JOURNAL OF MEDICINAL PLANTS[Internet]. 2009;8(29):100-106. Available from: https://sid.ir/paper/15228/en

IEEE: Copy

T. GHAZANFARI, ROYA YARAEE, A. ASOUDEH, T. RAJABIAN, M. KARDAR, ZOHEYR MOHAMMAD HASAN, H. NADERIMANESH, S. SOUFIAN, and D. JAMALI, “PURIFICATION OF IMMUNOMODULATOR FRACTION COMPONENTS OF GARLIC (R10) BY HPLC,” JOURNAL OF MEDICINAL PLANTS, vol. 8, no. 29, pp. 100–106, 2009, [Online]. Available: https://sid.ir/paper/15228/en

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