ELICITATION EFFECTS OF FUNGAL EXTRACTS ON SILYMARIN ACCUMULATION IN SILYBUM MARIANUM (L.) GAERTN HAIRY ROOT CULTURE

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

HASANLOO T.; AHMADI M.; KHAYYAM NEKOUEI S.M.; SALEHI JOUZANI GH.R.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Medicinal Plants Year:2013 | Volume:12 | Issue:48 Page(s): 25-39

Download Full-Text

Persian Verion

View:

1,212

Download:

Cites:

Information Journal Paper

Title

ELICITATION EFFECTS OF FUNGAL EXTRACTS ON SILYMARIN ACCUMULATION IN SILYBUM MARIANUM (L.) GAERTN HAIRY ROOT CULTURE

Author(s)

HASANLOO T. | AHMADI M. | KHAYYAM NEKOUEI S.M. | SALEHI JOUZANI GH.R. | Issue Writer Certificate

Keywords

SILYBUM MARIANUMQ3

ELICTATIONQ2

FUNGAL EXTRACTQ2

HAIRY ROOTQ3

SILYMARINQ2

Abstract

Background: SILYMARIN, extracted from the seeds of milk thistle (SILYBUM MARIANUM (L.) Gaertn) is mostly used for liver disease treatment. HAIRY ROOT cultures derived from S. marianum are able to produce SILYMARIN.Objective: Elicitation of HAIRY ROOT cultures is an important strategy for improving the production of secondary metabolites. The elicitors could be changed metabolite biosynthesis pathway and are useful for study of cell signaling pathway.Methods: In this study after preparation of S. marianum HAIRY ROOT cultures, the effects of various levels of Fusarium oxysprum and Phytophtora meloni extract (0, 10 and 20 mg 50 ml-1 culture) in 4 different exposure times (0, 24, 48 and 72 h) have been investigated on flavonolignans production. The flavonolignans were analyzed by High Performance Liquid Chromatography method.Results: Our results showed that HAIRY ROOT cultures of S. marianum were consisted of a large number of flavonolignans including silychristin, silydianin, silybin, isosilybin and taxifolin. The highest production of SILYMARIN (0.32 mg g-1 DW) was observed in F. oxysprum elicited root cultures (10 mg/50 ml culture) after 72h (2.28- fold that of the control). In Ph. meloni treated root cultures (20 mg/50 ml culture), the maximum SILYMARIN production (0.13 mg g-1 DW) was obtained after 72 h (1.9- fold that of the control).Conclusion: In this experiment it has been concluded that HAIRY ROOT cultures of S. marianum are susceptible to elicitation by fungal elicitors and is useful for efficient large-scale production of SILYMARIN by HAIRY ROOT cultures of S. marianum.

Cites

No record.

References

Cite

APA: Copy

HASANLOO, T., AHMADI, M., KHAYYAM NEKOUEI, S.M., & SALEHI JOUZANI, GH.R.. (2013). ELICITATION EFFECTS OF FUNGAL EXTRACTS ON SILYMARIN ACCUMULATION IN SILYBUM MARIANUM (L.) GAERTN HAIRY ROOT CULTURE. JOURNAL OF MEDICINAL PLANTS, 12(48), 25-39. SID. https://sid.ir/paper/15456/en

Vancouver: Copy

HASANLOO T., AHMADI M., KHAYYAM NEKOUEI S.M., SALEHI JOUZANI GH.R.. ELICITATION EFFECTS OF FUNGAL EXTRACTS ON SILYMARIN ACCUMULATION IN SILYBUM MARIANUM (L.) GAERTN HAIRY ROOT CULTURE. JOURNAL OF MEDICINAL PLANTS[Internet]. 2013;12(48):25-39. Available from: https://sid.ir/paper/15456/en

IEEE: Copy

T. HASANLOO, M. AHMADI, S.M. KHAYYAM NEKOUEI, and GH.R. SALEHI JOUZANI, “ELICITATION EFFECTS OF FUNGAL EXTRACTS ON SILYMARIN ACCUMULATION IN SILYBUM MARIANUM (L.) GAERTN HAIRY ROOT CULTURE,” JOURNAL OF MEDICINAL PLANTS, vol. 12, no. 48, pp. 25–39, 2013, [Online]. Available: https://sid.ir/paper/15456/en

Related Journal Papers