EFFECTS OF DIFFERENT CONCENTRATIONS OF DMSO AND GLYCEROL ON CRYOPRESERVATION OF TRICHOMONAS GALLINAE

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TAVASOLI MOUSA; JAVADI SHAHRAM; NAEM S.; VAHED GH.

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Journal: Iranian Journal of Veterinary Medicine Year:2009 | Volume:3 | Issue:2 Page(s): 83-86

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Title

EFFECTS OF DIFFERENT CONCENTRATIONS OF DMSO AND GLYCEROL ON CRYOPRESERVATION OF TRICHOMONAS GALLINAE

Author(s)

TAVASOLI MOUSA | JAVADI SHAHRAM | NAEM S. | VAHED GH. | Issue Writer Certificate

Keywords

CRYOPRESERVATIONQ3

TRICHOMONAS GALLINAEQ3

DMSOQ3

GLYCEROLQ3

Abstract

Conventional methods for the propagation and preservation of parasites in vivo or in vitro have limitations, which include the need for labor, the initial isolation and loss of strains, bacterial and fungal contamination, and changes in the original biological and metabolic characteristics of the organism. All of these disadvantages are reduced considerably by CRYOPRESERVATION. In this study, we examined the effects of different concentrations of dimethyl sulfoxide (DMSO) and GLYCEROL on the survival rate of Both of the two cryoprotectants examined at different concentrations showed a protective effect of T. gallinae When DMSO was used as the cryoprotectant, a maximum survival rate (70%) was achieved at a concentration of 10% over a short-term duration of freezing (24 h). GLYCEROL at a concentration of 10% had the highest effect on cryoprotectivity after 70 days, while DMSO 20% showed the lowest CRYOPRESERVATION T. gallinae. For in conclusion, DMSO and GLYCEROL are appropriate protective materials for the CRYOPRESERVATION of T. gallinae. The solutions of DMSO 10% and GLYCEROL 10% could be the best choice of cryoprotectant for short-term (24 hours) and long-term (70 days) protection, respectively.

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APA: Copy

TAVASOLI, MOUSA, JAVADI, SHAHRAM, NAEM, S., & VAHED, GH.. (2009). EFFECTS OF DIFFERENT CONCENTRATIONS OF DMSO AND GLYCEROL ON CRYOPRESERVATION OF TRICHOMONAS GALLINAE. IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH), 3(2), 83-86. SID. https://sid.ir/paper/157944/en

Vancouver: Copy

TAVASOLI MOUSA, JAVADI SHAHRAM, NAEM S., VAHED GH.. EFFECTS OF DIFFERENT CONCENTRATIONS OF DMSO AND GLYCEROL ON CRYOPRESERVATION OF TRICHOMONAS GALLINAE. IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH)[Internet]. 2009;3(2):83-86. Available from: https://sid.ir/paper/157944/en

IEEE: Copy

MOUSA TAVASOLI, SHAHRAM JAVADI, S. NAEM, and GH. VAHED, “EFFECTS OF DIFFERENT CONCENTRATIONS OF DMSO AND GLYCEROL ON CRYOPRESERVATION OF TRICHOMONAS GALLINAE,” IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH), vol. 3, no. 2, pp. 83–86, 2009, [Online]. Available: https://sid.ir/paper/157944/en

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