Detection and Molecular Characterization of Avian Rotaviruses in Broiler Farms in Guilan

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Poursafar Fatemeh; KARIMI VAHID; GHALYANCHI LANGEROUDI ARASH; HOSSEINI HOSSEIN; MOUSAVI FATEMEH SADAT

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Journal: Iranian Journal of Veterinary Medicine Year:2019 | Volume:13 | Issue:1 Page(s): 27-36

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Title

Detection and Molecular Characterization of Avian Rotaviruses in Broiler Farms in Guilan

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Keywords

Avian rotavirusesQ1

Broiler chickensQ1

IranQ2

NSP4 geneQ1

Phylogenetic studyQ1

Abstract

BACKGROUND: Avian rotaviruses are one of the important agents of enteritis in birds and have a worldwide distribution. These viruses have an important role in runting and stunting syndrome in chickens. OBJECTIVES: The aim of this study was detection and molecular characterization of Avian rotaviruses in broiler farms in Guilan province. METHODS: In this study, 100 samples were collected on different farms from the intestines of healthy and diseased Broiler chickens and tested by RT-PCR targeting of the NSP4 gene. Sequencing of NSP4 gene has been used for characterization of detected viruses. RESULTS: Avian rotaviruses were detected in 46% of samples by RT-PCR. Partial sequencing of seven NSP4 genes and phylogenetic analysis indicated that the viruses belonged to group A Avian rotaviruses and were 97. 52% to 100% homologous. Sequence identity between viruses with previous chicken rotavirus sequences was 31. 68% to 99. 5%. In comparison with turkey rotavirus Ty-1 and pigeon rotavirus, the homology was 76. 98% to 78. 22% and 74. 75% to 76. 98%, respectively. Evident differences were detected between the rotaviruses studied and human and pig rotaviruses with homologies of 31. 19% to 38. 86% for pig and 47. 77% to 48. 02% for human viruses. CONCLUSIONS: This study was the first molecular characterization of Avian rotaviruses in Iran. Results of this study showed that the group A Avian rotaviruses are one of the considerable enteric pathogens in Broiler chickens of Guilan province. Further study on the whole genome structure could elucidate the evolutionary process of Avian rotaviruses circulating in Iran.

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APA: Copy

Poursafar, Fatemeh, KARIMI, VAHID, GHALYANCHI LANGEROUDI, ARASH, HOSSEINI, HOSSEIN, & MOUSAVI, FATEMEH SADAT. (2019). Detection and Molecular Characterization of Avian Rotaviruses in Broiler Farms in Guilan. IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH), 13(1 ), 27-36. SID. https://sid.ir/paper/158040/en

Vancouver: Copy

Poursafar Fatemeh, KARIMI VAHID, GHALYANCHI LANGEROUDI ARASH, HOSSEINI HOSSEIN, MOUSAVI FATEMEH SADAT. Detection and Molecular Characterization of Avian Rotaviruses in Broiler Farms in Guilan. IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH)[Internet]. 2019;13(1 ):27-36. Available from: https://sid.ir/paper/158040/en

IEEE: Copy

Fatemeh Poursafar, VAHID KARIMI, ARASH GHALYANCHI LANGEROUDI, HOSSEIN HOSSEINI, and FATEMEH SADAT MOUSAVI, “Detection and Molecular Characterization of Avian Rotaviruses in Broiler Farms in Guilan,” IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH), vol. 13, no. 1 , pp. 27–36, 2019, [Online]. Available: https://sid.ir/paper/158040/en

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