BIOCHEMICAL AND MOLECULAR ANALYSIS OF GLUTAMINE SYNTHETASE GENE IN BRASSICA NAPUS L.

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JALALI JAVARAN MOKHTAR; MORRIS K.; BUCHANAN WOLLASTON V.

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Journal: Iran Agricultural Research Year:2001 | Volume:20 | Issue:1 Page(s): 17-36

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Title

BIOCHEMICAL AND MOLECULAR ANALYSIS OF GLUTAMINE SYNTHETASE GENE IN BRASSICA NAPUS L.

Author(s)

JALALI JAVARAN MOKHTAR | MORRIS K. | BUCHANAN WOLLASTON V. | Issue Writer Certificate

Keywords

BRASSICA NAPUS L.Q3

GLUTAMINE SYNTHETASEQ3

LEAF SENESCENCEQ3

PLANT DEVELOPMENT.Q3

Abstract

Leaf senescence represents a developmental process that recycles valuable nutrient elements for other parts of the plants. The combined action of several internal and external signals may be involved in the induction of senescence. From a practical viewpoint, delaying senescence may result in major economic benefits. Macromolecular changes that occur during LEAF SENESCENCE in BRASSICA NAPUS L. were investigated as a first step in studying this process. In this study, the changes in the level of GLUTAMINE SYNTHETASE (OS) enzyme in BRASSICA NAPUS L.aves during development were measured. Total OS activity was measured in soluble proteins isolated from leaves at different developmental stages. The level of OS activity in the leaf decreased during senescence. The behavior of the cytosolic (OSI) and chloroplastic (OS2) isoforms of GLUTAMINE SYNTHETASE was examined in the naturally senescing leaves of Brassica napus by western blotting. In the senescence stages the amount of OS1 appeared greater than OS2, indicating that the GS2 isoform declined during senescence and the GSI increased. The level of GSI expression increased in BRASSICA NAPUS L.aves during PLANT DEVELOPMENT.and reached maximum levels, during senescence at the SS3 stage. To study the GSI encoded by LSC460 at the molecular and biochemical level, doublestranded sequence was obtained from the LSC460 cDNA clone and compared with sequences in the databases. A search in available GenBank database revealed high sequence similarities of LSC460 to families of GLUTAMINE SYNTHETASE enzymes (especially with GSI). We conclude that the GS gene expressed during LEAF SENESCENCE of Brassica napus encoded by LSC460 is a GSI gene. These results show the importance of the GSI gene in senescence of the BRASSICA NAPUS L.aves.

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APA: Copy

JALALI JAVARAN, MOKHTAR, MORRIS, K., & BUCHANAN WOLLASTON, V.. (2001). BIOCHEMICAL AND MOLECULAR ANALYSIS OF GLUTAMINE SYNTHETASE GENE IN BRASSICA NAPUS L.. IRAN AGRICULTURAL RESEARCH, 20(1), 17-36. SID. https://sid.ir/paper/16093/en

Vancouver: Copy

JALALI JAVARAN MOKHTAR, MORRIS K., BUCHANAN WOLLASTON V.. BIOCHEMICAL AND MOLECULAR ANALYSIS OF GLUTAMINE SYNTHETASE GENE IN BRASSICA NAPUS L.. IRAN AGRICULTURAL RESEARCH[Internet]. 2001;20(1):17-36. Available from: https://sid.ir/paper/16093/en

IEEE: Copy

MOKHTAR JALALI JAVARAN, K. MORRIS, and V. BUCHANAN WOLLASTON, “BIOCHEMICAL AND MOLECULAR ANALYSIS OF GLUTAMINE SYNTHETASE GENE IN BRASSICA NAPUS L.,” IRAN AGRICULTURAL RESEARCH, vol. 20, no. 1, pp. 17–36, 2001, [Online]. Available: https://sid.ir/paper/16093/en

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