USE OF MICROSATELLITE ASSOCIATED WITH BODY WEIGHT IN SCREENING OF RAINBOW TROUT BROOD STOCK POPULATION (ONCORHYNCHUS MYKISS)

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GHOVATI N.; FARAHMAND H.; RAFIEE GH.; MIRVAGHEFI A.; MOHAMMADTAHERI M.; KHALILI B.

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Journal: Journal of Fisheries Year:2010 | Volume:63 | Issue:1 Page(s): 19-28

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Title

USE OF MICROSATELLITE ASSOCIATED WITH BODY WEIGHT IN SCREENING OF RAINBOW TROUT BROOD STOCK POPULATION (ONCORHYNCHUS MYKISS)

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Keywords

RAINBOW TROUTQ2

MICROSATELLITEQ2

BODY WEIGHTQ3

OLIGOSCREENINGQ3

MARKER ASSISTED SELECTION (MAS)Q3

Abstract

The aim of present study was to screen RAINBOW TROUT broodstock population of Kelardasht reproduction and rearing center, with emphasis on presence and non-presence of MICROSATELLITE markers associated with BODY WEIGHT. For this purpose, 92 samples of caudal fin tissue from Kelardasht RAINBOW TROUT broodstock population were obtained and genomic DNA of these samples were extracted. Three MICROSATELLITE loci include omyRGT1TUF (linkage group 5), omyRGT4TUF (linkage group Oi) and omyRGT14TUF (linkage group N) were amplified using the polymerase chain reaction (PCR) with their specific primers. This study showed that presence of these MICROSATELLITE markers in this broodstock population were 92.4%, 78.8% and 96.97% and the number of polymorph for those were 6, 4 and 6 respectively. In 25% of high weight broodstocks there was significant relationship between presence of omyRGT4TUF (p<0.05) and complete association between omyRGT14TUF with BODY WEIGHT in females as well as complete association between omyRGT1TUF and omyRGT14TUF with BODY WEIGHT in males.

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APA: Copy

GHOVATI, N., FARAHMAND, H., RAFIEE, GH., MIRVAGHEFI, A., MOHAMMADTAHERI, M., & KHALILI, B.. (2010). USE OF MICROSATELLITE ASSOCIATED WITH BODY WEIGHT IN SCREENING OF RAINBOW TROUT BROOD STOCK POPULATION (ONCORHYNCHUS MYKISS). JOURNAL OF FISHERIES (IRANIAN JOURNAL OF NATURAL RESOURCES), 63(1), 19-28. SID. https://sid.ir/paper/161961/en

Vancouver: Copy

GHOVATI N., FARAHMAND H., RAFIEE GH., MIRVAGHEFI A., MOHAMMADTAHERI M., KHALILI B.. USE OF MICROSATELLITE ASSOCIATED WITH BODY WEIGHT IN SCREENING OF RAINBOW TROUT BROOD STOCK POPULATION (ONCORHYNCHUS MYKISS). JOURNAL OF FISHERIES (IRANIAN JOURNAL OF NATURAL RESOURCES)[Internet]. 2010;63(1):19-28. Available from: https://sid.ir/paper/161961/en

IEEE: Copy

N. GHOVATI, H. FARAHMAND, GH. RAFIEE, A. MIRVAGHEFI, M. MOHAMMADTAHERI, and B. KHALILI, “USE OF MICROSATELLITE ASSOCIATED WITH BODY WEIGHT IN SCREENING OF RAINBOW TROUT BROOD STOCK POPULATION (ONCORHYNCHUS MYKISS),” JOURNAL OF FISHERIES (IRANIAN JOURNAL OF NATURAL RESOURCES), vol. 63, no. 1, pp. 19–28, 2010, [Online]. Available: https://sid.ir/paper/161961/en

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