EFFECT OF MORPHINE-SENSITIZATION IN D2 RECEPTOR GENE EXPRESSION IN THE MICE BRAIN IN THE ABSENCE AND PRESENCE OF LITHIUM CHLORIDE

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MEHREGAN HODA; SADEGHIZADEH MAJID; ZARINDAST M.R.; AZADMANESH K.; JAHANSHAHI ALI; SHIRZAD H.; SAADATI MOJTABA

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Journal: Cell Journal (Yakhteh) Year:2010 | Volume:12 | Issue:3 (47) Page(s): 395-404

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Title

EFFECT OF MORPHINE-SENSITIZATION IN D2 RECEPTOR GENE EXPRESSION IN THE MICE BRAIN IN THE ABSENCE AND PRESENCE OF LITHIUM CHLORIDE

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Keywords

MORPHINE SENSITIZATIONQ1

D2 RECEPTORQ1

LITHIUM CHLORIDEQ1

REAL-TIME PCRQ1

GENE EXPRESSIONQ1

Abstract

Objective: In this study we have investigated the changes in D2 RECEPTOR expression level in morphine-sensitized mice, in the absence and presence of LITHIUM CHLORIDE (LiCl). The result would pave the way to comprehend and confront this complicated event.Materials and Methods: Male NMRI mice, weighing 20-25g, were used in this study. They were divided into six groups. The first group received 0.9% saline as the control group and the other group was treated with morphine sulphate (30 mg/kg). LiCl (5 and 10 mg/kg treatments) was separately performed in two other groups. The final two groups were simultaneously treated with morphine sulphate (30 mg/kg) and LiCl (5 mg/kg) in one group and morphine sulphate (30 mg/kg) accompanied by LiCl (10 mg/kg) in the other group. All injections were performed intraperitoneally and once daily. After a five day wash-out, mice were decapitated and the brain regions which included the striatum, prefrontal cortex (PFC) and hippocampus were extracted. Using relative Real-Time polymerase chain reaction (PCR), the expression levels of the long (D2L) and short (D2S) isoforms of the D2 RECEPTOR were investigated.Results: Morphine treatment leads to a significant increase (p<0.0.5) in D2S levels in the striatum and PFC but has no effect on D2L levels in the examined regions. In the group receiving LiCl 5mg/kg, the D2L levels showed a significant augmentation in PFC and the hippocampus (p<0.05) as well as the striatum (p<0.001). The D2S levels in the same group, significantly increased in the PFC (p<0.05) and striatum (p<0.001). LiCl at a dose of 10 mg/kg did not alter the expression of either isoforms in any region. While simultaneous administration of morphine and LiCl (10 mg/kg) resulted in a marked increase in D2S levels in the striatum (p<0.001) and PFC (p<0.05), morphine administration along with LiCl (5mg/kg) was ineffective on the expression levels of D2L and D2S isoforms when compared to the control group.Conclusion: MORPHINE SENSITIZATION leads to an increase in D2S receptor expression and is affected by lithium in a dose-dependent manner where the lower dose inhibits and higher dose to enhances this effect. It is assumed that sensitization-induced changes in the transcript level are more regulated by dopamine transmission rather than by the direct effect of morphine and/or lithium on GENE EXPRESSION.

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APA: Copy

MEHREGAN, HODA, SADEGHIZADEH, MAJID, ZARINDAST, M.R., AZADMANESH, K., JAHANSHAHI, ALI, SHIRZAD, H., & SAADATI, MOJTABA. (2010). EFFECT OF MORPHINE-SENSITIZATION IN D2 RECEPTOR GENE EXPRESSION IN THE MICE BRAIN IN THE ABSENCE AND PRESENCE OF LITHIUM CHLORIDE. CELL JOURNAL (YAKHTEH), 12(3 (47)), 395-404. SID. https://sid.ir/paper/16298/en

Vancouver: Copy

MEHREGAN HODA, SADEGHIZADEH MAJID, ZARINDAST M.R., AZADMANESH K., JAHANSHAHI ALI, SHIRZAD H., SAADATI MOJTABA. EFFECT OF MORPHINE-SENSITIZATION IN D2 RECEPTOR GENE EXPRESSION IN THE MICE BRAIN IN THE ABSENCE AND PRESENCE OF LITHIUM CHLORIDE. CELL JOURNAL (YAKHTEH)[Internet]. 2010;12(3 (47)):395-404. Available from: https://sid.ir/paper/16298/en

IEEE: Copy

HODA MEHREGAN, MAJID SADEGHIZADEH, M.R. ZARINDAST, K. AZADMANESH, ALI JAHANSHAHI, H. SHIRZAD, and MOJTABA SAADATI, “EFFECT OF MORPHINE-SENSITIZATION IN D2 RECEPTOR GENE EXPRESSION IN THE MICE BRAIN IN THE ABSENCE AND PRESENCE OF LITHIUM CHLORIDE,” CELL JOURNAL (YAKHTEH), vol. 12, no. 3 (47), pp. 395–404, 2010, [Online]. Available: https://sid.ir/paper/16298/en

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