A STUDY OF THE MAJOR COMPONENTS OF THE PLURINET NETWORK EXPRESSION IN DENTAL PULP STEM CELLS ISOLATED FROM HUMAN THIRD MOLAR TEETH

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EBRAHIMI BEHNAM; YAGHOUBI M.M.; ABBASNEZHAD MAHDI; MOHAMMADI KAMALABADI A.; RAOUF M.

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Journal: Cell Journal (Yakhteh) Year:2010 | Volume:12 | Issue:3 (47) Page(s): 349-356

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Title

A STUDY OF THE MAJOR COMPONENTS OF THE PLURINET NETWORK EXPRESSION IN DENTAL PULP STEM CELLS ISOLATED FROM HUMAN THIRD MOLAR TEETH

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Keywords

PLURIPOTENCYQ1

STEM CELL MARKERQ1

GENE EXPRESSIONQ1

DENTAL PULPQ1

Abstract

Objective: The objective of this study was to isolate and culture human DENTAL PULP stem cells to study important STEM CELL MARKERs in them.Materials and Methods: Dental stem cells were isolated from human pulp and cultured in alpha-modified eagle’s medium (a-MEM) supplemented with 20% fetal bovine serum (FBS) in a 37oC incubator with 5% CO2 and photographed under inverted microscope. The expressions of the important STEM CELL MARKERs were analyzed by reverse transcription polymerase chain reaction (RT-PCR) and agarose gel electrophoresis in the cells at different passages.Results: Cells isolated from DENTAL PULP showed a high rate of proliferation and were cultured to more than 15 passages in vitro. The study of GENE EXPRESSION by RT-PCR showed that these cells expressed nucleostemin, cyclin D1, Oct-4 and nanog (major components of the PluriNet) in different passages as well as under serum-free conditions.Conclusion: Cells isolated from DENTAL PULP are genuine pluripotent stem cells with high potential for self-renewal. The expression of the STEM CELL MARKERs in human DENTAL PULP stem cells indicate that they have a great potential for cell therapy and regenerative medicine, even they were isolated from adult teeth.

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APA: Copy

EBRAHIMI, BEHNAM, YAGHOUBI, M.M., ABBASNEZHAD, MAHDI, MOHAMMADI KAMALABADI, A., & RAOUF, M.. (2010). A STUDY OF THE MAJOR COMPONENTS OF THE PLURINET NETWORK EXPRESSION IN DENTAL PULP STEM CELLS ISOLATED FROM HUMAN THIRD MOLAR TEETH. CELL JOURNAL (YAKHTEH), 12(3 (47)), 349-356. SID. https://sid.ir/paper/16309/en

Vancouver: Copy

EBRAHIMI BEHNAM, YAGHOUBI M.M., ABBASNEZHAD MAHDI, MOHAMMADI KAMALABADI A., RAOUF M.. A STUDY OF THE MAJOR COMPONENTS OF THE PLURINET NETWORK EXPRESSION IN DENTAL PULP STEM CELLS ISOLATED FROM HUMAN THIRD MOLAR TEETH. CELL JOURNAL (YAKHTEH)[Internet]. 2010;12(3 (47)):349-356. Available from: https://sid.ir/paper/16309/en

IEEE: Copy

BEHNAM EBRAHIMI, M.M. YAGHOUBI, MAHDI ABBASNEZHAD, A. MOHAMMADI KAMALABADI, and M. RAOUF, “A STUDY OF THE MAJOR COMPONENTS OF THE PLURINET NETWORK EXPRESSION IN DENTAL PULP STEM CELLS ISOLATED FROM HUMAN THIRD MOLAR TEETH,” CELL JOURNAL (YAKHTEH), vol. 12, no. 3 (47), pp. 349–356, 2010, [Online]. Available: https://sid.ir/paper/16309/en

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