ISOLATION, CULTURE AND CHARACTERIZATION OF HUMAN SYNOVIUM-DERIVED MESENCHYMAL STEM CELLS

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KADIVAR MAHDI; DARVISH MARYAM; SALEHI MOGHADAM MASOUD

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Journal: Cell Journal (Yakhteh) Year:2009 | Volume:11 | Issue:2 (42) Page(s): 160-167

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Information Journal Paper

Title

ISOLATION, CULTURE AND CHARACTERIZATION OF HUMAN SYNOVIUM-DERIVED MESENCHYMAL STEM CELLS

Author(s)

KADIVAR MAHDI | DARVISH MARYAM | SALEHI MOGHADAM MASOUD | Issue Writer Certificate

Keywords

MESENCHYMAL STEM CELLSQ1

KNEEQ1

SYNOVIUMQ1

TISSUEQ1

Abstract

Objective: It has been demonstrated that MESENCHYMAL STEM CELLS (MSCs), which are isolated from various TISSUEs, have different potential in differentiation and proliferation. For this reason it is necessary to isolate these cells from various sources in order to use them in clinical settings. The present study has been done to investigate the possibility of isolation, culture and characterization of human SYNOVIUM-derived MESENCHYMAL STEM CELLS.Materials and Methods: Samples (200-300mg) were provided from SYNOVIUM TISSUE of patients who had KNEE surgery. Obtained samples were homogenized, enzymatically minced with collagenase D and passed through 70mm nylon filters; separated cells were then cultured. Isolated cells were identified by morphological observations, differentiation tests, flow cytometry, immunocytochemistery studies and RT-PCR.Results: The isolated cells in this study showed fibroblast-like morphology and have a high proliferation capacity. In flow cytometry and immunocytochemical studies, they were positive for CD73 and CD105 antigens. RT-PCR analysis and specific staining for differentiated cells towards osteogenic and adipogenic lineages, showed that isolated cells were potent in differentiation into the mentioned lineages.Conclusion: These results suggest that SYNOVIUM TISSUE, which is discarded in most KNEE operations, can be used for cell therapy and TISSUE engineering protocols as an enrichment source of potent MESENCHYMAL STEM CELLS.

Cites

COMPARISON OF THE DIFFERENTIATION POTENTIAL OF HUMAN MESENCHYMAL STEM CELLS AND SEVERAL ANIMAL SPECIES

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APA: Copy

KADIVAR, MAHDI, DARVISH, MARYAM, & SALEHI MOGHADAM, MASOUD. (2009). ISOLATION, CULTURE AND CHARACTERIZATION OF HUMAN SYNOVIUM-DERIVED MESENCHYMAL STEM CELLS. CELL JOURNAL (YAKHTEH), 11(2 (42)), 160-167. SID. https://sid.ir/paper/16415/en

Vancouver: Copy

KADIVAR MAHDI, DARVISH MARYAM, SALEHI MOGHADAM MASOUD. ISOLATION, CULTURE AND CHARACTERIZATION OF HUMAN SYNOVIUM-DERIVED MESENCHYMAL STEM CELLS. CELL JOURNAL (YAKHTEH)[Internet]. 2009;11(2 (42)):160-167. Available from: https://sid.ir/paper/16415/en

IEEE: Copy

MAHDI KADIVAR, MARYAM DARVISH, and MASOUD SALEHI MOGHADAM, “ISOLATION, CULTURE AND CHARACTERIZATION OF HUMAN SYNOVIUM-DERIVED MESENCHYMAL STEM CELLS,” CELL JOURNAL (YAKHTEH), vol. 11, no. 2 (42), pp. 160–167, 2009, [Online]. Available: https://sid.ir/paper/16415/en

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