INVESTIGATION OF NEOGOBIUS GORLAP POPULATION GENETIC STRUCTURE IN COASTS OF MAXANDARAN AND GOLESTAN PROVINCES BY MICROSATELLITE MARKERS

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POURGHOLAM H.; ZAMINI A.A.; LALOUEI F.; KHARA H.; TAGHAVI M.J.

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Journal: JOURNAL OF BIOLOGY SCIENCE Year:2011 | Volume:5 | Issue:2 (SERIES NO. 17) Page(s): 33-44

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Title

INVESTIGATION OF NEOGOBIUS GORLAP POPULATION GENETIC STRUCTURE IN COASTS OF MAXANDARAN AND GOLESTAN PROVINCES BY MICROSATELLITE MARKERS

Author(s)

Keywords

GENETIC VARIATIONQ3

MICROSATELLITEQ2

CASPIAN SEAQ3

METAPENAEUS AFFINISQ2

Abstract

Genetic diversity of Neogobios gorlap poplution were examined on 100 samples from regions in southern of the CASPIAN SEA (Mazandaran and Golestan regions). Genomic DNA was extracted from fin tissue by phenol-chlorophorm method. PCR was performed using MICROSATELLITE primers. The PCR products of samples were accompanied with standard marker (50 bp lader). To measure fragment size samples were run on a 8% polyacrylamid gel. Allel count and frequency, the effective and real allel, expected heterozygosity and observed heterozygosity, genetic diversity, shannon Information index, genetic identity and genetic distance, F ST, RST, gene flow and Hardy-Weinberg Equilibrium based on Analysis of Molecular Variance (AMOVA) were caculated using the Gene Alex software. The mean of observed and effective allel number was 12.41 and 8.02 respectively and also the mean of observed and expected heterozygosity was 0.695 and 0.845 respectively. It was also seen that specimens from all regions (exception locus NG71, NG111 and NG215 in Golestan region) were not in Hardy- Weinberg Equibrium in all of the loci (P<0.001). Bassed on Analysis of Molcular Variance (AMOVA)F ST was 0.028 between Golestan and Mazandaran regions. Also Nm was 8.726 between Golestan and Mazandaran regions. Genetic distance was 0.354 between specimens from Mazandaran and Golestan regions. As result, there is a significant genetic divergence between some of samples (P<0.01). Therefore, two genetic group of Neogobios gorlapwere identified in Southern part of the CASPIAN SEA (Mazandaran and Golestan regions).

Cites

ژنتیک جمعیت ماهی شبه شوریده دهان سیاه Atrobucca nibe) Jordan and Thompson, 1911) در سواحل شمال غربی دریای عمان با استفاده از روش PCR-sequencing

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APA: Copy

POURGHOLAM, H., ZAMINI, A.A., LALOUEI, F., KHARA, H., & TAGHAVI, M.J.. (2011). INVESTIGATION OF NEOGOBIUS GORLAP POPULATION GENETIC STRUCTURE IN COASTS OF MAXANDARAN AND GOLESTAN PROVINCES BY MICROSATELLITE MARKERS. JOURNAL OF BIOLOGY SCIENCE, 5(2 (SERIES NO. 17)), 33-44. SID. https://sid.ir/paper/164171/en

Vancouver: Copy

POURGHOLAM H., ZAMINI A.A., LALOUEI F., KHARA H., TAGHAVI M.J.. INVESTIGATION OF NEOGOBIUS GORLAP POPULATION GENETIC STRUCTURE IN COASTS OF MAXANDARAN AND GOLESTAN PROVINCES BY MICROSATELLITE MARKERS. JOURNAL OF BIOLOGY SCIENCE[Internet]. 2011;5(2 (SERIES NO. 17)):33-44. Available from: https://sid.ir/paper/164171/en

IEEE: Copy

H. POURGHOLAM, A.A. ZAMINI, F. LALOUEI, H. KHARA, and M.J. TAGHAVI, “INVESTIGATION OF NEOGOBIUS GORLAP POPULATION GENETIC STRUCTURE IN COASTS OF MAXANDARAN AND GOLESTAN PROVINCES BY MICROSATELLITE MARKERS,” JOURNAL OF BIOLOGY SCIENCE, vol. 5, no. 2 (SERIES NO. 17), pp. 33–44, 2011, [Online]. Available: https://sid.ir/paper/164171/en

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